PLK1
Serine/threonine-protein kinase PLK1 (EC 2.7.11.21) (Polo-like kinase 1) (PLK-1) (Serine/threonine-protein kinase 13) (STPK13) [PLK]
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The storage of surplus oocytes by cryopreservation (OC) is a widely used tool in assisted reproductive technology, but there is a great debate about the effects of cryopreservation on oocyte competence. It is known that OC may affect meiotic spindles but remains unclear if OC may increase the risk of aneuploidy. The aim of this study was to test the effects of OC and women aging on the expression of cytokinesis-related genes playing an important role in the segregation of chromosomes (DCTN1, DCTN2, DCTN3, DCTN6 and PLK1). Results highlighted that OC do not modify the expression of the selected genes, whereas women aging modulate the expression of all transcripts, confirming that aging is the crucial factor affecting meiosis and aneuploidy risk. A new role for Dynactin and PLK1 was shed in light, providing information on the ageing process in the oocyte which may be associated to reduced fertility.
MeSH Terms
- Adult
- Age Factors
- Aging
- Aneuploidy
- Cryopreservation
- Dynactin Complex
- Gene Expression
- Gene Expression Regulation
- Humans
- Oocyte Retrieval
- Oocytes
- Real-Time Polymerase Chain Reaction
- Reproductive Techniques, Assisted
- Vitrification
Keywords
- Aging
- Aneuploidy
- Cryopreservation
- Dynactin
- Gene expression
- Oocyte
Polo-like kinase 1 (PLK1) plays a key role in various stages of mitosis from entry into M phase to exit from mitosis. However, its role in cellular senescence remains to be determined. Therefore, the effects of PLK1 on cellular senescence in human primary cells were investigated. We found that expression of PLK1 decreased in human dermal fibroblasts and human umbilical vein endothelial cells under replicative senescence and premature senescence induced by adriamycin. PLK1 knockdown with PLK1 small interfering RNAs in young cells induced premature senescence. In contrast, upregulation of PLK1 in old cells partially reversed senescence phenotypes. Cellular senescence by PLK1 inhibition was observed in p16 knockdown cells but not in p53 knockdown cells. Our data suggest that PLK1 repression might result in cellular senescence in human primary cells via a p53-dependent pathway.
MeSH Terms
- Cell Cycle Proteins
- Cells, Cultured
- Cellular Senescence
- Cyclin-Dependent Kinase Inhibitor p16
- Down-Regulation
- Gene Knockdown Techniques
- Genes, p16
- Genes, p53
- Human Umbilical Vein Endothelial Cells
- Humans
- Protein-Serine-Threonine Kinases
- Proto-Oncogene Proteins
- RNA, Messenger
- RNA, Small Interfering
- Signal Transduction
- Tumor Suppressor Protein p53
Keywords
- Cellular senescence
- Human primary cells.
- Polo-like kinase 1
- p53