DCTN3
Dynactin subunit 3 (Dynactin complex subunit 22 kDa subunit) (p22) [DCTN22]
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The storage of surplus oocytes by cryopreservation (OC) is a widely used tool in assisted reproductive technology, but there is a great debate about the effects of cryopreservation on oocyte competence. It is known that OC may affect meiotic spindles but remains unclear if OC may increase the risk of aneuploidy. The aim of this study was to test the effects of OC and women aging on the expression of cytokinesis-related genes playing an important role in the segregation of chromosomes (DCTN1, DCTN2, DCTN3, DCTN6 and PLK1). Results highlighted that OC do not modify the expression of the selected genes, whereas women aging modulate the expression of all transcripts, confirming that aging is the crucial factor affecting meiosis and aneuploidy risk. A new role for Dynactin and PLK1 was shed in light, providing information on the ageing process in the oocyte which may be associated to reduced fertility.
MeSH Terms
- Adult
- Age Factors
- Aging
- Aneuploidy
- Cryopreservation
- Dynactin Complex
- Gene Expression
- Gene Expression Regulation
- Humans
- Oocyte Retrieval
- Oocytes
- Real-Time Polymerase Chain Reaction
- Reproductive Techniques, Assisted
- Vitrification
Keywords
- Aging
- Aneuploidy
- Cryopreservation
- Dynactin
- Gene expression
- Oocyte
Sporadic amyotrophic lateral sclerosis (SALS) is a fatal motor neuron degenerative disease of unclear pathogenesis. Disturbances of intracellular transport are possible causes of the disease. We evaluated the expression of motor proteins involved in the anterograde (kinesins KIF1B, KIF5C) and retrograde (KIFC3, dynactin subunits DCTN1 and DCTN3) intracellular transport in peripheral blood mononuclear cells (PBMCs). PBMCs were obtained from 74 SALS patients with different clinical phenotypes, 65 blood donors (healthy control I), and 29 cases with other neurological diseases (disease control II) divided into subgroups IIA (atypical parkinsonism) and IIB (ALS-mimicking disorders). mRNA expression was studied by real-time qPCR, and protein level by Western blotting. In SALS, KIF5C and KIFC3 expression was significantly lower and DCTN1 higher than in control I, and dependent of age. KIF1B expression was significantly higher in SALS than in subgroup IIB, whereas DCTN1 and DCTN3 were higher in SALS than in subgroup IIA. All changes in the studied proteins were statistically significant in classic ALS but not in progressive muscular atrophy. In SALS, and especially in classic ALS, the changes in motor protein expression may alter bidirectional intracellular transport in PBMCs. More studies are needed to find out whether the levels of KIF5C and DCTN1 may be useful in ALS diagnosis, and whether KIF1B expression may discriminate ALS from ALS-mimicking disorders.
MeSH Terms
- Adult
- Aged
- Aged, 80 and over
- Aging
- Amyotrophic Lateral Sclerosis
- Biomarkers
- Blotting, Western
- Dynactin Complex
- Female
- Humans
- Kinesin
- Leukocytes, Mononuclear
- Male
- Middle Aged
- RNA, Messenger
- Real-Time Polymerase Chain Reaction
- Young Adult