DCTN2

Dynactin subunit 2 (50 kDa dynein-associated polypeptide) (Dynactin complex 50 kDa subunit) (DCTN-50) (p50 dynamitin) [DCTN50]

Publications

Dynactin pathway-related gene expression is altered by aging, but not by vitrification.

PubMed

Full text

The storage of surplus oocytes by cryopreservation (OC) is a widely used tool in assisted reproductive technology, but there is a great debate about the effects of cryopreservation on oocyte competence. It is known that OC may affect meiotic spindles but remains unclear if OC may increase the risk of aneuploidy. The aim of this study was to test the effects of OC and women aging on the expression of cytokinesis-related genes playing an important role in the segregation of chromosomes (DCTN1, DCTN2, DCTN3, DCTN6 and PLK1). Results highlighted that OC do not modify the expression of the selected genes, whereas women aging modulate the expression of all transcripts, confirming that aging is the crucial factor affecting meiosis and aneuploidy risk. A new role for Dynactin and PLK1 was shed in light, providing information on the ageing process in the oocyte which may be associated to reduced fertility.

MeSH Terms

• Adult
• Age Factors
• Aging
• Aneuploidy
• Cryopreservation
• Dynactin Complex
• Gene Expression
• Gene Expression Regulation
• Humans
• Oocyte Retrieval
• Oocytes
• Real-Time Polymerase Chain Reaction
• Reproductive Techniques, Assisted
• Vitrification

Keywords

• Aging
• Aneuploidy
• Cryopreservation
• Dynactin
• Gene expression
• Oocyte