EHF
ETS homologous factor (hEHF) (ETS domain-containing transcription factor) (Epithelium-specific Ets transcription factor 3) (ESE-3) [ESE3] [ESE3B] [ESEJ]
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Extended high frequencies (EHF), above 8 kHz, represent a region of the human hearing spectrum that is generally ignored by clinicians and researchers alike. This article is a compilation of contributions that, together, make the case for an essential role of EHF in both normal hearing and auditory dysfunction. We start with the fundamentals of biological and acoustic determinism - humans have EHF hearing for a purpose, for example, the detection of prey, predators, and mates. EHF hearing may also provide a boost to speech perception in challenging conditions and its loss, conversely, might help explain difficulty with the same task. However, it could be that EHF are a marker for damage in the conventional frequency region that is more related to speech perception difficulties. Measurement of EHF hearing in concert with otoacoustic emissions could provide an early warning of age-related hearing loss. In early life, when EHF hearing sensitivity is optimal, we can use it for enhanced phonetic identification during language learning, but we are also susceptible to diseases that can prematurely damage it. EHF audiometry techniques and standardization are reviewed, providing evidence that they are reliable to measure and provide important information for early detection, monitoring and possible prevention of hearing loss in populations at-risk. To better understand the full contribution of EHF to human hearing, clinicians and researchers can contribute by including its measurement, along with measures of speech in noise and self-report of hearing difficulties and tinnitus in clinical evaluations and studies.
Keywords
- Aging
- Development
- Extended high frequency audiometry
- Otitis media
- Ototoxicity
- Speech in noise
- Speech perception
- Tinnitus
The applying of millimeter-wave electromagnetic emission (EHF-therapy) is an effective method for various age-related pathologies treatment, among other cardio-vascular diseases. During the EHF-emission of aging human endothelial cell cultures it was obtained changing of NO-synthase (eNOS), endothelin-1, angiotensin-2 and vasopressin expression dependence of irradiation exposition. These data have shown that EHF-emission has activated endothelium functional activity, which can play the important role to search for approaches to treatment of arterial hypertension and atherosclerosis.
MeSH Terms
- Aging
- Cells, Cultured
- Dose-Response Relationship, Radiation
- Electromagnetic Fields
- Endothelium, Vascular
- Female
- Humans
- In Vitro Techniques
- Nitric Oxide
- Pregnancy
The influence of extreme radiofrequency millimeter microwave (EHF) and infrared (IR) electromagnetic emanation on the molecular markers of cell renovation (Ki67, p53) and proinflammatory cytokine TNF-α expression in the buccal cells of various age patients with chronic parodontitis was investigated. The results show that EHF- and IR-electromagnetic emanation increased Ki67 proliferative marker expression and decreased expression of proapoptosis protein p53 and proinflammatory cytokine TNF-α in the buccal epithelium of young, middle-aged and elderly people with chronic parodontitis. The data obtained open the new ability for patogenetic treatment of various age patients with chronic parodontitis using the EHF- and IR-electromagnetic emanation method.
MeSH Terms
- Aged
- Aged, 80 and over
- Aging
- Chronic Disease
- Chronic Periodontitis
- Combined Modality Therapy
- Humans
- Immunohistochemistry
- Infrared Rays
- Ki-67 Antigen
- Microwaves
- Middle Aged
- Mouth Mucosa
- Tumor Necrosis Factor-alpha
- Tumor Suppressor Protein p53
The cornea is the clear, outermost portion of the eye composed of three layers: an epithelium that provides a protective barrier while allowing transmission of light into the eye, a collagen-rich stroma, and an endothelium monolayer. How cornea development and aging is controlled is poorly understood. Here we characterize the mouse cornea transcriptome from early embryogenesis through aging and compare it with transcriptomes of other epithelial tissues, identifying cornea-enriched genes, pathways, and transcriptional regulators. Additionally, we profiled cornea epithelium and stroma, defining genes enriched in these layers. Over 10,000 genes are differentially regulated in the mouse cornea across the time course, showing dynamic expression during development and modest expression changes in fewer genes during aging. A striking transition time point for gene expression between postnatal days 14 and 28 corresponds with completion of cornea development at the transcriptional level. Clustering classifies co-expressed, and potentially co-regulated, genes into biologically informative categories, including groups that exhibit epithelial or stromal enriched expression. Based on these findings, and through loss of function studies and ChIP-seq, we show that the Ets transcription factor EHF promotes cornea epithelial fate through complementary gene activating and repressing activities. Furthermore, we identify potential interactions between EHF, KLF4, and KLF5 in promoting cornea epithelial differentiation. These data provide insights into the mechanisms underlying epithelial development and aging, identifying EHF as a regulator of cornea epithelial identity and pointing to interactions between Ets and KLF factors in promoting epithelial fate. Furthermore, this comprehensive gene expression data set for the cornea is a powerful tool for discovery of novel cornea regulators and pathways.
MeSH Terms
- Aging
- Animals
- Cell Differentiation
- Cell Lineage
- Cornea
- Embryonic Development
- Epithelial Cells
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Kruppel-Like Transcription Factors
- Mice
- Transcription Factors
Keywords
- Aging
- Chromatin Immunoprecipitation (ChiP)
- Cornea
- DNA Transcription
- Development
- Epithelium
- Eye
- Gene Expression
- Transcription/Developmental Factors
The aim of the present study was to investigate the effect of age of the sire on the in vitro quality of frozen-thawed (FT) bull spermatozoa, both when tested immediately postthaw (PT) and when assessed after cleansing and selection through a swim-up (SU) procedure. Semen samples from six Swedish Red and White Breed (SRB) artificial insemination (AI) bulls at age 1 and again, at 4 years were collected and frozen in 0.25 ml plastic straws. Also, semen was collected from six Estonian Holstein (EHF) bulls at the ages of 3, 5, and 7 years and likewise processed. The FT semen was tested for the susceptibility of sperm nuclear deoxyribonucleic acid (DNA) to undergo acid-induced denaturation in situ, as quantified by flow cytometry (FCM). The DNA denaturability was expressed as function alpha t, i.e., as the ratio of red (denaturated DNA) to red green (total cellular DNA) fluorescence intensity. The results were expressed as the percentage of cells with high alpha t values, i.e., cells outside the main population (% COMP alpha t). Morphological evaluation of the same samples was performed to detect general and sperm head abnormalities and differences between ages. Fertility results were available as non-return rates (NRRs) for the semen of the sires when they were 1 year (SRB) and 3 years (EHF) old, varying from 62.2 to 70.7% in SRB and from 52.2 to 76.0% in EHF animals. The COMP alpha t values ranged from 0.5-3.6% (PT) to 0.2-1.7% (SU) for SRB bulls and from 0.4-1.8% (PT) to 0.2-1.5% (SU) for EHF bulls. Both breeds lacked differences between ages, either PT or after SU. However, the SU procedure yielded a significantly higher population of spermatozoa with stable DNA following acid-induced denaturation, than PT samples (p < 0.001). No correlation was detected between field fertility and chromatin stability. The results indicate that for these bull populations, the SU procedure was able to select spermatozoa with stable chromatin from the bulk samples. However, the use of DNA denaturation as a challenge to assess sperm chromatin stability did not offer a more accurate tool to evaluate sperm quality than the conventional, light microscopical evaluation of morphology.
MeSH Terms
- Aging
- Animals
- Cattle
- Chromatin
- Cryopreservation
- DNA
- Drug Stability
- Fertility
- Hot Temperature
- Insemination, Artificial
- Male
- Nucleic Acid Denaturation
- Semen Preservation
- Sensitivity and Specificity
- Spermatozoa
Most measures of auditory sensitivity at extended high frequencies (frequencies greater than 8 kHz) have been obtained from listeners with normal hearing less than 40 years of age. The purpose of this study was (a) to measure thresholds at frequencies above 8 kHz in older listeners who, as a group, have elevated thresholds at lower frequencies, and (b) to assess test-retest reliability, age and gender effects, and the influence of thresholds below 8 kHz. Extended high-frequency (EHF) thresholds were measured for 162 older listeners (60-79 years) using a commercially available high-frequency audiometer, with a frequency range of 8 to 18 kHz and an intensity range of 0 to 110 dB SPL. Thresholds were measured once at the beginning of a 1- to 2-hour test session and then remeasured at the end of the test session. EHF thresholds of older listeners with normal hearing at conventional audiometric frequencies were substantially higher than the thresholds reported for younger listeners, with normal hearing by Dreschler and van der Hulst (1987). EHF thresholds of older listeners with hearing loss at conventional audiometric frequencies were further elevated as compared to older listeners with normal hearing. Differences in EHF thresholds between females and males were either not present or were reduced when gender differences in conventional audiometric thresholds were taken into account. No significant differences were seen in thresholds at 8 kHz and higher between the 60- to 69-and 70- to 79-year-old age groups. Results also indicated that thresholds above 8 kHz can be measured in older listeners within a clinically acceptable /- 10 dB test-retest range.
MeSH Terms
- Age Factors
- Aged
- Aging
- Female
- Hearing Disorders
- Humans
- Longitudinal Studies
- Male
- Middle Aged
- Sex Factors
The results presented in this report show that while bone marrow of intact donors transplanted to irradiated (10 Gy) recipient mice produces a 2.5-fold increase in the life span of animals, bone marrow of donors exposed to EHF increases it by 35 times.
MeSH Terms
- Animals
- Bone Marrow
- Bone Marrow Transplantation
- Dose-Response Relationship, Radiation
- Gamma Rays
- Longevity
- Mice
- Mice, Inbred C57BL
- Mice, Inbred CBA
- Radio Waves
- Time Factors
- Tissue Donors