Bone morphogenetic protein receptor type-1B precursor (EC 2.7.11.30) (BMP type-1B receptor) (BMPR-1B) (CDw293 antigen)
развернуть
Growth hormone during in vitro fertilization in older women modulates the density of receptors in granulosa cells, with improved pregnancy outcomes.
To study the effect of aging and granulosa cell growth hormone receptor (GHR) expression, and the effect of growth hormone (GH) co-treatment during IVF on receptor expression. Laboratory study. University. A total of 445 follicles were collected from 62 women undergoing standard infertility treatment. Preovulatory ovarian follicle biopsies of granulosa cells and follicular fluid. Older women with a poor ovarian reserve were co-treated with GH to determine the effect of the adjuvant during IVF on the granulosal expression density of FSH receptor (FSHR), LH receptor (LHR), bone morphogenetic hormone receptor (BMPR1B), and GHR. Ovarian reserve, granulosa cell receptor density, oocyte quality, and pregnancy and live birth rates were determined. Growth hormone co-treatment increased the receptor density for granulosal FSHR, BMPR1B, LHR, and GHR compared with the non-GH-treated patients of the same age and ovarian reserve. Growth hormone co-treatment increased GHR density, which may increase GHR activity. The GH co-treatment was associated with a significant increase in pregnancy rate. Growth hormone co-treatment restored the preovulatory down-regulation of FSHR, BMPR1B, and LHR density of the largest follicles, which may improve the maturation process of luteinization in older patients with reduced ovarian reserve. The fertility of the GH-treated patients improved.
MeSH Terms
- Adult
- Bone Morphogenetic Protein Receptors, Type I
- Drug Administration Schedule
- Female
- Fertilization in Vitro
- Granulosa Cells
- Human Growth Hormone
- Humans
- Infertility, Female
- Maternal Age
- Middle Aged
- Ovarian Reserve
- Pregnancy
- Pregnancy Outcome
- Pregnancy Rate
- Receptors, FSH
- Receptors, LH
- Receptors, Somatotropin
- Young Adult
Keywords
- Aging
- fertility
- growth hormone
- pregnancy rate
- receptor density
развернуть
Dysregulation of granulosal bone morphogenetic protein receptor 1B density is associated with reduced ovarian reserve and the age-related decline in human fertility.
Reproductive ageing is linked to the depletion of ovarian primordial follicles, which causes an irreversible change to ovarian cellular function and the capacity to reproduce. The current study aimed to profile the expression of bone morphogenetic protein receptor, (BMPR1B) in 53 IVF patients exhibiting different degrees of primordial follicle depletion. The granulosa cell receptor density was measured in 403 follicles via flow cytometry. A decline in BMPR1B density occurred at the time of dominant follicle selection and during the terminal stage of folliculogenesis in the 23-30 y good ovarian reserve patients. The 40 y poor ovarian reserve patients experienced a reversal of this pattern. The results demonstrate an association between age-induced depletion of the ovarian reserve and BMPR1B receptor density at the two critical time points of dominant follicle selection and pre-ovulatory follicle maturation. Dysregulation of BMP receptor signalling may inhibit the normal steroidogenic differentiation required for maturation in older patients.
MeSH Terms
- Adult
- Aging
- Bone Morphogenetic Protein Receptors, Type I
- Female
- Gene Expression Regulation
- Humans
- Infertility, Female
- Ovarian Follicle
- Ovarian Reserve
- Signal Transduction
- Young Adult
Keywords
- Bone morphogenetic protein receptor 1B
- Human granulosa cells
- Ovary
- Receptors