FGL2
Fibroleukin precursor (Fibrinogen-like protein 2) (pT49)
Publications[править]
Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5 B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5 and CD5 B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5 B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse.
MeSH Terms
- Aging
- Animals
- B-Lymphocyte Subsets
- B-Lymphocytes
- CD5 Antigens
- Flow Cytometry
- Horses
- Immunoglobulin Light Chains
- Immunophenotyping
- Lymphoid Tissue
- Phosphorylcholine
Keywords
- B cell
- CD5
- Development
- Equine
- Immunoglobulin repertoire