Редактирование: LRP2 (раздел)

==Publications==

{{medline-entry
|title=Single-Cell Transcriptomics Characterizes Cell Types in the Subventricular Zone and Uncovers Molecular Defects Impairing Adult Neurogenesis.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30485812
|abstract=Neural stem cells (NSCs) contribute to plasticity and repair of the adult brain. Niches harboring NSCs regulate stem cell self-renewal and differentiation. We used comprehensive and untargeted single-cell RNA profiling to generate a molecular cell atlas of the largest germinal region of the adult mouse brain, the subventricular zone (SVZ). We characterized >20 neural and non-neural cell types and gained insights into the dynamics of neurogenesis by predicting future cell states based on computational analysis of RNA kinetics. Furthermore, we applied our single-cell approach to document decreased numbers of NSCs, reduced proliferation activity of progenitors, and perturbations in Wnt and BMP signaling pathways in mice lacking [[LRP2]], an endocytic receptor required for SVZ maintenance. Our data provide a valuable resource to study adult neurogenesis and a proof of principle for the power of single-cell RNA sequencing to elucidate neural cell-type-specific alterations in loss-of-function models.
|mesh-terms=* Aging
* Animals
* Cell Lineage
* Cell Proliferation
* Dentate Gyrus
* Gene Expression Regulation
* Lateral Ventricles
* Low Density Lipoprotein Receptor-Related Protein-2
* Mice, Inbred C57BL
* Models, Biological
* Neural Stem Cells
* Neurogenesis
* RNA
* RNA, Long Noncoding
* Single-Cell Analysis
* Stem Cell Niche
* Transcriptome
|keywords=* Drop-seq
* LDL-receptor related protein 2
* RNA velocity
* adult neural stem cells
* adult neurogenesis
* neurogenic niche
* single-cell RNA sequencing
* subventricular zone
|full-text-url=https://sci-hub.do/10.1016/j.celrep.2018.11.003
}}