SELENOP
Selenoprotein P precursor (SeP) [SELP] [SEPP1]
Publications[править]
The hierarchies of tissue selenium distribution and selenotranscriptomes are thought to critically affect healthspan and longevity. We determined selenium status and selenotranscriptomes in response to long-term dietary selenium deficiency and age in tissues of male and female mice. Weanling telomerase RNA component knockout C57BL/6 mice were fed a selenium-deficient (0.03 mg Se/kg) Torula yeast-based AIN-93G diet or a diet supplemented with sodium selenate (0.15 mg Se/kg) until age 18 or 24 mo. Plasma, hearts, kidneys, livers, and testes were collected to assay for selenotranscriptomes, selected selenoproteins, and tissue selenium concentrations. Data were analyzed with the use of 2-factor ANOVA (diet × age) in both sexes. Dietary selenium deficiency decreased ([i]P[/i] ≤ 0.05) selenium concentrations (65-72%) and glutathione peroxidase (GPX) 3 (82-94%) and selenoprotein P (SELENOP) (17-41%) levels in the plasma of both sexes of mice and mRNA levels (9-68%) of 4, 4, and 12 selenoproteins in the heart, kidney, and liver of males, respectively, and 5, 16, and 14 selenoproteins, respectively, in females. Age increased selenium concentrations and SELENOP levels (27% and 30%, respectively; [i]P[/i] ≤ 0.05) in the plasma of males only but decreased (12-46%; [i]P[/i] < 0.05) mRNA levels of 1, 5, and 13 selenoproteins in the heart, kidney, and liver of males, respectively, and 6, 5, and 0 selenoproteins, respectively, in females. Among these mRNAs, selenoprotein H ([i]Selenoh[/i]), selenoprotein M ([i]Selenom[/i]), selenoprotein W ([i]Selenow[/i]), methionine-[i]R[/i]-sulfoxide reductase 1 ([i]MsrB1[/i]), [i]Gpx1[/i], [i]Gpx3[/i], thioredoxin reductase 1 ([i]Txnrd1[/i]), [i]Txnrd2[/i], selenoprotein S ([i]Selenos[/i]), selenoprotein F ([i]Selenof[/i]), and selenoprotein O ([i]Selenoo[/i]) responded in parallel to dietary selenium deficiency and age in ≥1 tissue or sex, or both. Dietary selenium deficiency upregulated (40-160%; [i]P[/i] ≤ 0.05) iodothyronine deiodinase 2 ([i]Dio2[/i]) and selenoprotein N ([i]Selenon[/i]) in the kidneys of males. Age upregulated (11-44%; [i]P[/i] < 0.05) [i]Selenon[/i] in the kidneys of males, selenoprotein K ([i]Selenok[/i]) and selenoprotein I ([i]Selenoi[/i]) in the kidneys of females, and [i]Selenof[/i] and [i]Selenok[/i] in the testes. These results illustrate tissue-specific sexual dimorphisms of selenium status and selenotranscriptomes because of dietary selenium deficiency and age.
MeSH Terms
- Age Factors
- Animals
- Deficiency Diseases
- Diet
- Female
- Gene Expression Profiling
- Glutathione Peroxidase
- Heart
- Kidney
- Liver
- Longevity
- Male
- Mice, Inbred C57BL
- Mice, Knockout
- Myocardium
- Oxidoreductases
- RNA, Messenger
- Selenium
- Selenoproteins
- Sex Factors
- Telomerase
- Telomere
- Testis
Keywords
- age
- metabolism
- selenium
- selenotranscriptome
- sex