MT1M
Metallothionein-1M (MT-1M) (Metallothionein-IM) (MT-IM) [MT1K]
Publications[править]
Twelve isolates isolated from the gastrointestinal tracts of Gaotian villagers in China, who had a lifespan of 92 yr, were examined for their antioxidants using free radical scavenging activity and 2,2-diphenyl-1-picrylhydrazyl. Three strains (i.e., Lactobacillus mucosae LMU1001, and Lactobacillus plantarum LPL0902 and LPL0302) were selected as candidates to prepare yogurt for testing their antioxidants in a model of d-galactose-induced aging mice, with vitamin C as a positive control. The results showed that L. mucosae LMU1001 was the best strain, which had similar in vivo antioxidant activity as vitamin C. A significant increase was found in the activities of glutathione peroxidase in serum and total superoxide dismutase in the liver, and a decrease in the level of malondialdehyde in serum. Regarding mRNA expression level detected quantitatively by real-time PCR, we observed that L. mucosae LMU1001 significantly upregulated antioxidant genes (i.e., MT1A and MT1M in HT-29 and Caco-2) and those genes (i.e., MT1, MT2, GPx1, and GPx2) in the intestinal tract of the model mice. Hence, this strain could be considered as a potential probiotic lactic acid bacterium for improving antioxidant levels in functional foods.
MeSH Terms
- Aged, 80 and over
- Aging
- Animals
- Antioxidants
- Caco-2 Cells
- China
- Functional Food
- Galactose
- Glutathione Peroxidase
- Humans
- Intestines
- Lactobacillus
- Lactobacillus plantarum
- Liver
- Longevity
- Malondialdehyde
- Mice
- Models, Animal
- Probiotics
- Superoxide Dismutase
Keywords
- 2,2-diphenyl-1-picrylhydrazyl
- antioxidant activity
- d-galactose-induced aging mice
- hydroxyl radical
- reducing power