Gamma-glutamyl hydrolase precursor (EC 220.127.116.11) (Conjugase) (GH) (Gamma-Glu-X carboxypeptidase)
A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.
- Chromatography, High Pressure Liquid
- Dansyl Compounds
- Indicators and Reagents
- Middle Aged
- Spectrometry, Fluorescence