Материал из hpluswiki
Перейти к навигации Перейти к поиску

Gamma-glutamyl hydrolase precursor (EC (Conjugase) (GH) (Gamma-Glu-X carboxypeptidase)


Pre-column derivatization and its optimum conditions for quantitative determination of urinary hydroxylysine glycosides by high-performance liquid chromatography.

A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.

MeSH Terms

  • Adult
  • Aged
  • Aging
  • Chromatography, High Pressure Liquid
  • Dansyl Compounds
  • Glycosides
  • Humans
  • Hydroxylysine
  • Indicators and Reagents
  • Middle Aged
  • Spectrometry, Fluorescence
  • Stereoisomerism