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Cysteine-rich secretory protein 2 precursor (CRISP-2) (Cancer/testis antigen 36) (CT36) (Testis-specific protein TPX-1) [GAPDL5] [TPX1]


Novel development-related alternative splices in human testis identified by cDNA microarrays.

Alternative splicing of premessenger RNA is an important regulatory mechanism that increases the diversity of proteins transcribed from a single gene. This is particularly important in the testis because germ cell expansion and differentiation require many cellular changes and regulatory steps. To investigate novel development-related alternative splicings in the human testis, complementary DNA microarray studies were conducted with the use of probes from human fetal testes, adult testes, and human spermatozoa. Of a total of 386 Unigene clusters found to be related to the development of the testis, 67 clusters showed a total of 74 novel alternative spliceoforms. Developmental stage-dependent expression was also performed for a novel Unigene, NYD-SP20 (Hs.351068), which had 4 possible novel spliceoforms and another Unigene, CRISP2 (cysteine-rich secretory protein 2, Hs.2042), which had 3 possible novel spliceoforms. These results indicate that alternative splicing plays an important role in the complicated processes of testis development and spermatogenesis.

MeSH Terms

  • Adult
  • Aged
  • Aging
  • Alternative Splicing
  • Base Sequence
  • DNA Primers
  • DNA, Complementary
  • Gene Expression Regulation, Developmental
  • Humans
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Proteins
  • RNA, Messenger
  • Spermatozoa
  • Testis