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Expression profiling of cell cycle genes in human pancreatic islets with and without type 2 diabetes.

Microarray gene expression data were used to analyze the expression pattern of cyclin, cyclin-dependent kinase (CDKs) and cyclin-dependent kinase inhibitor (CDKIs) genes from human pancreatic islets with and without type 2 diabetes (T2D). Of the cyclin genes, CCNI was the most expressed. Data obtained from microarray and qRT-PCR showed higher expression of CCND1 in diabetic islets. Among the CDKs, CDK4, CDK8 and CDK9 were highly expressed, while CDK1 was expressed at low level. High expression of [[CDK18]] was observed in diabetic islets. Of the CDKIs, CDKN1A expression was higher in diabetic islets in both microarray and qRT-PCR. Expression of CDKN1A, [[CDKN2A]], CCNI2, CDK3 and CDK16 was correlated with age. Finally, eight SNPs in these genes were associated with T2D in the DIAGRAM database. Our data provide a comprehensive expression pattern of cell cycle genes in human islets. More human studies are required to confirm and reproduce animal studies.

MeSH Terms

  • Aged
  • Aging
  • Case-Control Studies
  • Cells, Cultured
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclin-Dependent Kinases
  • Cyclins
  • Diabetes Mellitus, Type 2
  • Female
  • Gene Expression Profiling
  • Genes, cdc
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Humans
  • Insulin
  • Insulin Secretion
  • Islets of Langerhans
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Polymorphism, Single Nucleotide
  • Transcriptome