NALCN
Sodium leak channel non-selective protein (CanIon) (Voltage gated channel-like protein 1) [VGCNL1]
Publications[править]
The resting membrane potential of the pacemaker neurons is one of the essential mechanisms underlying rhythm generation. In this study, we described the biophysical properties of an uncharacterized channel (U-type channel) and investigated the role of the channel in the rhythmic activity of a respiratory pacemaker neuron and the respiratory behaviour in adult freshwater snail Lymnaea stagnalis. Our results show that the channel conducts an inward leak current carried by Na( ) (I(Leak-Na)). The I(Leak-Na) contributed to the resting membrane potential and was required for maintaining rhythmic action potential bursting activity of the identified pacemaker RPeD1 neurons. Partial knockdown of the U-type channel suppressed the aerial respiratory behaviour of the adult snail in vivo. These findings identified the Na( ) leak conductance via the U-type channel, likely a NALCN-like channel, as one of the fundamental mechanisms regulating rhythm activity of pacemaker neurons and respiratory behaviour in adult animals.
MeSH Terms
- Aging
- Amino Acid Sequence
- Animals
- Behavior, Animal
- Biological Clocks
- Calcium
- Cell Membrane
- Cell Separation
- Electricity
- Extracellular Space
- Gadolinium
- Gene Knockdown Techniques
- Ion Channel Gating
- Lymnaea
- Membrane Potentials
- Molecular Sequence Data
- Neurons
- RNA, Double-Stranded
- Sequence Alignment
- Sodium Channels