Metallothionein-1M (MT-1M) (Metallothionein-IM) (MT-IM) [MT1K]

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A novel strain of Lactobacillus mucosae isolated from a Gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in D-galactose-induced aging mice.

Twelve isolates isolated from the gastrointestinal tracts of Gaotian villagers in China, who had a lifespan of 92 yr, were examined for their antioxidants using free radical scavenging activity and 2,2-diphenyl-1-picrylhydrazyl. Three strains (i.e., Lactobacillus mucosae LMU1001, and Lactobacillus plantarum LPL0902 and LPL0302) were selected as candidates to prepare yogurt for testing their antioxidants in a model of d-galactose-induced aging mice, with vitamin C as a positive control. The results showed that L. mucosae LMU1001 was the best strain, which had similar in vivo antioxidant activity as vitamin C. A significant increase was found in the activities of glutathione peroxidase in serum and total superoxide dismutase in the liver, and a decrease in the level of malondialdehyde in serum. Regarding mRNA expression level detected quantitatively by real-time PCR, we observed that L. mucosae LMU1001 significantly upregulated antioxidant genes (i.e., MT1A and MT1M in HT-29 and Caco-2) and those genes (i.e., MT1, MT2, GPx1, and GPx2) in the intestinal tract of the model mice. Hence, this strain could be considered as a potential probiotic lactic acid bacterium for improving antioxidant levels in functional foods.

MeSH Terms

  • Aged, 80 and over
  • Aging
  • Animals
  • Antioxidants
  • Caco-2 Cells
  • China
  • Functional Food
  • Galactose
  • Glutathione Peroxidase
  • Humans
  • Intestines
  • Lactobacillus
  • Lactobacillus plantarum
  • Liver
  • Longevity
  • Malondialdehyde
  • Mice
  • Models, Animal
  • Probiotics
  • Superoxide Dismutase

Keywords

  • 2,2-diphenyl-1-picrylhydrazyl
  • antioxidant activity
  • d-galactose-induced aging mice
  • hydroxyl radical
  • reducing power