TAF1
Transcription initiation factor TFIID subunit 1 (EC 2.3.1.48) (EC 2.7.11.1) (Cell cycle gene 1 protein) (TBP-associated factor 250 kDa) (p250) (Transcription initiation factor TFIID 250 kDa subunit) (TAF(II)250) (TAFII-250) (TAFII250) [BA2R] [CCG1] [CCGS] [TAF2A]
Publications[править]
TATA-box binding protein associated factor 1 (TAF1) protein is the largest and the essential component of the TFIID complex in the pathway of RNA polymerase II-mediated gene transcription, and it regulates transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1), which we reported previously, may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. In the present study, we cloned the full-length cDNA that encodes the mouse homologue of N-TAF1 (N-Taf1) protein. By carrying out of real time RT-PCR, we investigated the expression analysis of the N-Taf1 mRNA in mouse tissues and cell lines. As well as the human N-TAF1, the N-Taf1 showed limited expression in the brain and neuroblastoma, whereas Taf1 expressed elsewhere. Furthermore, in mouse embryo head or mouse brain, mRNA expression of TAF1 changes dramatically during development but N-Taf1 showed sustained expression. Our result suggests that the N-Taf1 gene has an important role in non-dividing neuronal cell rather than in cell division and proliferation during neurogenesis.
MeSH Terms
- Aging
- Animals
- Base Sequence
- Brain
- Cell Division
- Cell Line, Tumor
- Cell Proliferation
- Cloning, Molecular
- Embryo, Mammalian
- Exons
- Gene Expression Regulation, Developmental
- Head
- Histone Acetyltransferases
- Humans
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Neurogenesis
- Neurons
- Open Reading Frames
- Protein Isoforms
- RNA, Messenger
- TATA-Binding Protein Associated Factors
- Transcription Factor TFIID