COL4A6
Collagen alpha-6(IV) chain precursor
Publications[править]
Little is known about mechanisms regulating gene expression for the alpha chains of basement membrane type IV collagen, arranged head-to-head in transcription units COL4A1-COL4A2, COL4A3-COL4A4, and COL4A5-COL4A6, and implicated broadly in genetic diseases. To investigate these mechanisms, we generated transgenic mouse lines bearing 5'-flanking sequences of COL4A5 and COL4A6, cloned upstream of a lacZ reporter gene. A 3.8-kb fragment upstream of COL4A6 directs reporter gene expression in the esophagus, stomach, and duodenum, whereas a 13.8-kb fragment directs expression in the esophagus only. A 10.6-kb fragment upstream of COL4A5 directs expression in the esophagus. Coupled with evidence of long-range conservation between human and mouse non-coding sequences, described herein, our findings provide the first indication that highly specialized patterns characteristic of COL4A5-COL4A6 expression in vivo arise from effects of distributed cis-acting regulatory elements on a bidirectional proximal promoter, itself transcriptionally competent.
MeSH Terms
- Aging
- Amino Acid Sequence
- Animals
- Collagen Type IV
- Gene Expression Regulation
- Genes, Regulator
- Humans
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Organ Specificity
- Protein Subunits
- Sequence Homology
- Species Specificity
- Tissue Distribution
- Transcriptional Activation
- Upper Gastrointestinal Tract