OOEP
Oocyte-expressed protein homolog (KH homology domain-containing protein 2) (Oocyte- and embryo-specific protein 19) (hOEP19) [C6orf156] [KHDC2] [OEP19]
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DNA damage in oocytes can cause infertility and birth defects. DNA double-strand breaks (DSBs) are highly deleterious and can substantially impair genome integrity. Homologous recombination (HR)-mediated DNA DSB repair plays dominant roles in safeguarding oocyte quantity and quality. However, little is known regarding the key players of the HR repair pathway in oocytes. Here, we identified oocyte-specific gene [i]Ooep[/i] as a novel key component of the HR repair pathway in mouse oocytes. OOEP was required for efficient ataxia telangiectasia mutated (ATM) kinase activation and Rad51 recombinase(RAD51)focal accumulation at DNA DSBs. [i]Ooep[/i] null oocytes were defective in DNA DSB repair and prone to apoptosis upon exogenous DNA damage insults. Moreover, [i]Ooep[/i] null oocytes exhibited delayed meiotic maturation. Therefore, OOEP played roles in preserving oocyte quantity and quality by maintaining genome stability. [i]Ooep[/i] expression decreased with the advance of maternal age, suggesting its involvement in maternal aging.
MeSH Terms
- Aging
- Animals
- DNA Breaks, Double-Stranded
- DNA Repair
- Female
- Meiosis
- Mice
- Mice, Inbred C57BL
- Oocytes
- RNA-Binding Proteins
- Recombination, Genetic
Keywords
- ATM
- DNA double-strand break repair
- Homologous recombination
- Ooep
- RAD51