MT1M

Metallothionein-1M (MT-1M) (Metallothionein-IM) (MT-IM) [MT1K]

Publications

A novel strain of Lactobacillus mucosae isolated from a Gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in D-galactose-induced aging mice.

PubMed

Full text

Twelve isolates isolated from the gastrointestinal tracts of Gaotian villagers in China, who had a lifespan of 92 yr, were examined for their antioxidants using free radical scavenging activity and 2,2-diphenyl-1-picrylhydrazyl. Three strains (i.e., Lactobacillus mucosae LMU1001, and Lactobacillus plantarum LPL0902 and LPL0302) were selected as candidates to prepare yogurt for testing their antioxidants in a model of d-galactose-induced aging mice, with vitamin C as a positive control. The results showed that L. mucosae LMU1001 was the best strain, which had similar in vivo antioxidant activity as vitamin C. A significant increase was found in the activities of glutathione peroxidase in serum and total superoxide dismutase in the liver, and a decrease in the level of malondialdehyde in serum. Regarding mRNA expression level detected quantitatively by real-time PCR, we observed that L. mucosae LMU1001 significantly upregulated antioxidant genes (i.e., MT1A and MT1M in HT-29 and Caco-2) and those genes (i.e., MT1, MT2, GPx1, and GPx2) in the intestinal tract of the model mice. Hence, this strain could be considered as a potential probiotic lactic acid bacterium for improving antioxidant levels in functional foods.

MeSH Terms

• Aged, 80 and over
• Aging
• Animals
• Antioxidants
• Caco-2 Cells
• China
• Functional Food
• Galactose
• Glutathione Peroxidase
• Humans
• Intestines
• Lactobacillus
• Lactobacillus plantarum
• Liver
• Longevity
• Malondialdehyde
• Mice
• Models, Animal
• Probiotics
• Superoxide Dismutase

Keywords

• 2,2-diphenyl-1-picrylhydrazyl
• antioxidant activity
• d-galactose-induced aging mice
• hydroxyl radical
• reducing power