LRP2

Low-density lipoprotein receptor-related protein 2 precursor (LRP-2) (Glycoprotein 330) (gp330) (Megalin)

PublicationsПравить

Single-Cell Transcriptomics Characterizes Cell Types in the Subventricular Zone and Uncovers Molecular Defects Impairing Adult Neurogenesis.

PubMed

Full text

Neural stem cells (NSCs) contribute to plasticity and repair of the adult brain. Niches harboring NSCs regulate stem cell self-renewal and differentiation. We used comprehensive and untargeted single-cell RNA profiling to generate a molecular cell atlas of the largest germinal region of the adult mouse brain, the subventricular zone (SVZ). We characterized >20 neural and non-neural cell types and gained insights into the dynamics of neurogenesis by predicting future cell states based on computational analysis of RNA kinetics. Furthermore, we applied our single-cell approach to document decreased numbers of NSCs, reduced proliferation activity of progenitors, and perturbations in Wnt and BMP signaling pathways in mice lacking LRP2, an endocytic receptor required for SVZ maintenance. Our data provide a valuable resource to study adult neurogenesis and a proof of principle for the power of single-cell RNA sequencing to elucidate neural cell-type-specific alterations in loss-of-function models.

MeSH Terms

Aging
Animals
Cell Lineage
Cell Proliferation
Dentate Gyrus
Gene Expression Regulation
Lateral Ventricles
Low Density Lipoprotein Receptor-Related Protein-2
Mice, Inbred C57BL
Models, Biological
Neural Stem Cells
Neurogenesis
RNA
RNA, Long Noncoding
Single-Cell Analysis
Stem Cell Niche
Transcriptome

Keywords

Drop-seq
LDL-receptor related protein 2
RNA velocity
adult neural stem cells
adult neurogenesis
neurogenic niche
single-cell RNA sequencing
subventricular zone