GGH

Gamma-glutamyl hydrolase precursor (EC 3.4.19.9) (Conjugase) (GH) (Gamma-Glu-X carboxypeptidase)

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Pre-column derivatization and its optimum conditions for quantitative determination of urinary hydroxylysine glycosides by high-performance liquid chromatography.

PubMed

Full text

A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.

MeSH Terms

Adult
Aged
Aging
Chromatography, High Pressure Liquid
Dansyl Compounds
Glycosides
Humans
Hydroxylysine
Indicators and Reagents
Middle Aged
Spectrometry, Fluorescence
Stereoisomerism