FDXR
NADPH:adrenodoxin oxidoreductase, mitochondrial precursor (EC 1.18.1.6) (AR) (Adrenodoxin reductase) (Ferredoxin--NADP( ) reductase) (Ferredoxin reductase) [ADXR]
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To explore associated proteins involved in age-related changes of the testis and better understand the roles of these proteins in the human testis. We used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spec trometry analysis to identify differentially expressed proteins between the aged and the normal control groups. The L-lactate dehydrogenase C chain (LDHC) protein, a previous testis-specific protein, was found to be downregulated in the aged testis and was further tested with western blot and immunohistochemical analysis to verify the result of the LDHC protein in 2-DE. Twelve differentially expressed proteins were identified. Among those proteins, 3 were upregulated and 9 were downregulated in the aged group. The results of western blot and immunohistochemical analysis confirmed the expression of LDHC downregulation in the aged testis. Some proteins identified had little well-known function in the human testis, as follows: AKR7A3, FDXR, PGAM1, SEPT2 and HMGCS2. Our results imply that the aged testis can be a good model to find associated proteins involved in age-related changes of the testis. It can be useful to understand the roles of those proteins in the testis.
MeSH Terms
- Aged
- Aging
- Down-Regulation
- Electrophoresis, Gel, Two-Dimensional
- Humans
- Immunohistochemistry
- Isoenzymes
- L-Lactate Dehydrogenase
- Male
- Middle Aged
- Proteins
- Proteomics
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Testis
- Young Adult