FGL2

Fibroleukin precursor (Fibrinogen-like protein 2) (pT49)

Publications

Developmental expression of B cell molecules in equine lymphoid tissues.

PubMed

Full text

Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5 B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5 and CD5 B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5 B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse.

MeSH Terms

• Aging
• Animals
• B-Lymphocyte Subsets
• B-Lymphocytes
• CD5 Antigens
• Flow Cytometry
• Horses
• Immunoglobulin Light Chains
• Immunophenotyping
• Lymphoid Tissue
• Phosphorylcholine

Keywords

• B cell
• CD5
• Development
• Equine
• Immunoglobulin repertoire