CRISP2
Cysteine-rich secretory protein 2 precursor (CRISP-2) (Cancer/testis antigen 36) (CT36) (Testis-specific protein TPX-1) [GAPDL5] [TPX1]
PublicationsПравить
Alternative splicing of premessenger RNA is an important regulatory mechanism that increases the diversity of proteins transcribed from a single gene. This is particularly important in the testis because germ cell expansion and differentiation require many cellular changes and regulatory steps. To investigate novel development-related alternative splicings in the human testis, complementary DNA microarray studies were conducted with the use of probes from human fetal testes, adult testes, and human spermatozoa. Of a total of 386 Unigene clusters found to be related to the development of the testis, 67 clusters showed a total of 74 novel alternative spliceoforms. Developmental stage-dependent expression was also performed for a novel Unigene, NYD-SP20 (Hs.351068), which had 4 possible novel spliceoforms and another Unigene, CRISP2 (cysteine-rich secretory protein 2, Hs.2042), which had 3 possible novel spliceoforms. These results indicate that alternative splicing plays an important role in the complicated processes of testis development and spermatogenesis.
MeSH Terms
- Adult
- Aged
- Aging
- Alternative Splicing
- Base Sequence
- DNA Primers
- DNA, Complementary
- Gene Expression Regulation, Developmental
- Humans
- Male
- Oligonucleotide Array Sequence Analysis
- Proteins
- RNA, Messenger
- Spermatozoa
- Testis