SLC39A14

Metal cation symporter ZIP14 precursor (LIV-1 subfamily of ZIP zinc transporter 4) (LZT-Hs4) (Solute carrier family 39 member 14) (Zrt- and Irt-like protein 14) (ZIP-14) [KIAA0062] [ZIP14]

Publications

Age and dietary iron affect expression of genes involved in iron acquisition and homeostasis in young pigs.

PubMed

Full text

To investigate the effects of dietary iron (Fe) and age on Fe metabolism, we used 36 weaned barrows in a 2 x 3 design with 2 concentrations of dietary Fe [97 (control) and 797 (high Fe) mg Fe/kg dry matter] and 3 time points of tissue collection (after 21, 42, or 63 d on diets). Pigs were weighed and bled on d 0, 20, 41, and 62. High Fe reduced feed efficiency but did not affect pig weight gain. Blood hemoglobin concentrations and Fe concentrations of liver, intestine, and heart were increased by high dietary Fe on all days. Concentrations of liver and heart Fe increased with age. As determined by quantitative real-time PCR, hepatic expression of hepcidin (HAMP) in pigs given the high-Fe diet was 6.25-fold that of control pigs. In the intestine, relative mRNA levels of ferroportin, divalent metal transporter 1, and transferrin receptor were downregulated by high Fe. Expression of an alternative route of Fe absorption, solute carrier family 39 member 14 (SLC39A14), was downregulated in the intestine of pigs fed high dietary Fe. Additionally, duodenal mRNA level of certain genes including scavenger receptor class A, member 5, and frataxin decreased with age of the animal. Our findings indicate new roles in Fe metabolism for several mineral metabolism-associated genes and that some of these genes, such as SLC39A14, may be regulated in response to dietary Fe in pigs. Additionally, the expression of some genes examined in this study was affected by age, suggesting age dependency of Fe metabolism in pigs.

MeSH Terms

• Aging
• Animals
• Antimicrobial Cationic Peptides
• Cation Transport Proteins
• Down-Regulation
• Duodenum
• Gene Expression
• Hemoglobins
• Hepcidins
• Homeostasis
• Intestinal Absorption
• Intestinal Mucosa
• Iron
• Iron, Dietary
• Iron-Binding Proteins
• Liver
• Male
• Myocardium
• RNA, Messenger
• Receptors, Transferrin
• Reverse Transcriptase Polymerase Chain Reaction
• Scavenger Receptors, Class A
• Swine