GGH

Gamma-glutamyl hydrolase precursor (EC 3.4.19.9) (Conjugase) (GH) (Gamma-Glu-X carboxypeptidase)

Publications

Pre-column derivatization and its optimum conditions for quantitative determination of urinary hydroxylysine glycosides by high-performance liquid chromatography.

PubMed

Full text

A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.

MeSH Terms

• Adult
• Aged
• Aging
• Chromatography, High Pressure Liquid
• Dansyl Compounds
• Glycosides
• Humans
• Hydroxylysine
• Indicators and Reagents
• Middle Aged
• Spectrometry, Fluorescence
• Stereoisomerism