https://transhumanist.ru/index.php?action=history&feed=atom&title=NKX2-5 2026-06-23T00:38:26Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=NKX2-5&diff=4601&oldid=prev 2021-04-29T19:25:57Z

<p>Новая страница: «Homeobox protein Nkx-2.5 (Cardiac-specific homeobox) (Homeobox protein CSX) (Homeobox protein NK-2 homolog E) [CSX] [NKX2.5] [NKX2E] ==Publications== {{medline-...»</p> <p><b>Новая страница</b></p><div>Homeobox protein Nkx-2.5 (Cardiac-specific homeobox) (Homeobox protein CSX) (Homeobox protein NK-2 homolog E) [CSX] [NKX2.5] [NKX2E]<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart.<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30663680<br /> |abstract=Myocardial infarction (MI) is a leading cause of morbidity and mortality around the world. A major goal of regenerative medicine is to replenish the dead myocardium after MI. Although several strategies have been used to regenerate myocardium, stem cell therapy remains a major approach to replenish the dead myocardium of an MI heart. Accumulating evidence suggests the presence of resident cardiac stem cells (CSCs) in the adult heart and their endocrine and/or paracrine effects on cardiac regeneration. However, CSC isolation and their characterization and differentiation toward myocardial cells, especially cardiomyocytes, remains a technical challenge. In the present study, we provided a simple method for the isolation, characterization, and differentiation of CSCs from the adult mouse heart. Here, we describe a density gradient method for the isolation of CSCs, where the heart is digested by a 0.2% collagenase II solution. To characterize the isolated CSCs, we evaluated the expression of CSCs/cardiac markers Sca-1, [[NKX2-5]], and [[GATA4]], and pluripotency/stemness markers OCT4, [[SOX2]], and Nanog. We also determined the proliferation potential of isolated CSCs by culturing them in a Petri dish and assessing the expression of the proliferation marker Ki-67. For evaluating the differentiation potential of CSCs, we selected seven- to ten-days cultured CSCs. We transferred them to a new plate with a cardiomyocyte differentiation medium. They are incubated in a cell culture incubator for 12 days, while the differentiation medium is changed every three days. The differentiated CSCs express cardiomyocyte-specific markers: actinin and troponin I. Thus, CSCs isolated with this protocol have stemness and cardiac markers, and they have a potential for proliferation and differentiation toward cardiomyocyte lineage.<br /> |mesh-terms=* Aging<br /> * Animals<br /> * Biomarkers<br /> * Cell Culture Techniques<br /> * Cell Differentiation<br /> * Male<br /> * Mice<br /> * Mice, Inbred C57BL<br /> * Multipotent Stem Cells<br /> * Myocardium<br /> * Myocytes, Cardiac<br /> <br /> |full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207148<br /> }}</div>

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