https://transhumanist.ru/index.php?action=history&feed=atom&title=MAN2B2 2026-05-16T01:44:54Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=MAN2B2&diff=5232&oldid=prev 2021-05-12T13:35:14Z

<p>Новая страница: «Epididymis-specific alpha-mannosidase precursor (EC 3.2.1.24) (Alpha-1,6-mannosidase) (Mannosidase alpha class 2B member 2) [KIAA0935] ==Publications== {{medlin...»</p> <p><b>Новая страница</b></p><div>Epididymis-specific alpha-mannosidase precursor (EC 3.2.1.24) (Alpha-1,6-mannosidase) (Mannosidase alpha class 2B member 2) [KIAA0935]<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=Refinement of the expression pattern of a mouse homologue of the porcine 135-kDa alpha-d-mannosidase ([[MAN2B2]]).<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/10860856<br /> |abstract=In the article by Hiramoto et al. (1997) (Stage-specific expression of a mouse homologue of the porcine 135 kDa alpha-d-mannosidase ([[MAN2B2]]) in type A spermatogonia, Biochem. Biophys. Res. Commun. 241, 439-445) a new homologue to the porcine epididymis-specific 135 kDa alpha-d-mannosidase ([[MAN2B2]]) was cloned from a mouse testis cDNA library. Northern blot analysis with RNA of different tissues showed a testis-specific expression by using a mouse [[MAN2B2]] oligonucleotide. In situ hybridization revealed that the mouse [[MAN2B2]] transcript is localized exclusively in spermatogonia. In consequence to this it was proposed by Hiramoto et al. that the mouse [[MAN2B2]] homologue could serve as a marker for spermatogonia. By repeating the published experiments we observed a different expression pattern of the mouse [[MAN2B2]] gene. Northern blot analysis with either testicular RNA from prepubertal males or with testicular RNA from the W/W(v)-mutant mouse which lacks germ cells showed an expression of the [[MAN2B2]] gene. Furthermore, Northern blot analysis with RNA from different somatic tissues revealed that the gene is ubiquitously expressed. Therefore, our refinement clearly demonstrates that the [[MAN2B2]] mouse homologue is not specifically expressed in spermatogonia.<br /> |mesh-terms=* Aging<br /> * Animals<br /> * Blotting, Northern<br /> * DNA Probes<br /> * Gene Expression Profiling<br /> * Gene Expression Regulation, Developmental<br /> * Male<br /> * Mannosidases<br /> * Mice<br /> * Mice, Mutant Strains<br /> * Molecular Weight<br /> * Organ Specificity<br /> * RNA, Messenger<br /> * Reproducibility of Results<br /> * Sequence Homology<br /> * Sexual Maturation<br /> * Spermatozoa<br /> * Swine<br /> * Testis<br /> * alpha-Mannosidase<br /> <br /> |full-text-url=https://sci-hub.do/10.1006/bbrc.2000.2868<br /> }}</div>

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