https://transhumanist.ru/index.php?action=history&feed=atom&title=HPR 2026-04-23T10:28:15Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=HPR&diff=5391&oldid=prev 2021-05-12T13:41:57Z

<p>Новая страница: «Haptoglobin-related protein ==Publications== {{medline-entry |title=Development of a microplate ELISA for circulating E-selectin: assay characterization, compar...»</p> <p><b>Новая страница</b></p><div>Haptoglobin-related protein<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=Development of a microplate ELISA for circulating E-selectin: assay characterization, comparison with a commercial kit, wand establishment of normal reference values.<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/12696081<br /> |abstract=E-selectin, an adhesion molecule, is detectable in the blood. Serum levels of E-selectin can be a prognostic indicator for various malignancies. We developed a microplate enzyme-linked immunosorbent assay (ELISA) for the detection of circulating E-selectin by coating the microwell with monoclonal anti-E-selectin. We incubated the specimen and the biotinylated detecting antibody simultaneously, and used [[HPR]]-conjugated streptavidin to generate a signal for quantification. The assay has a sensitivity of 3.8 ng/mL, and the coefficients of variation (CVs) for both within-day and day-to-day precision were all &lt;10%. Our in-house-made kit also compared well with a commercial kit from R</div>

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