https://transhumanist.ru/index.php?action=history&feed=atom&title=FOXO3B 2026-06-10T20:30:14Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=FOXO3B&diff=5635&oldid=prev 2021-05-12T13:53:08Z

<p>Новая страница: «box protein O3B ==Publications== {{medline-entry |title=Genetic investigation of FOXO3A requires special attention due to sequence homology with <a href="/FOXO3B" title="FOXO3B">FOXO3B</a>. |pu...»</p> <p><b>Новая страница</b></p><div>box protein O3B<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=Genetic investigation of FOXO3A requires special attention due to sequence homology with [[FOXO3B]].<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/22588664<br /> |abstract=Our study demonstrates that the genetic investigation of forkhead box O3A gene (FOXO3A), a validated human longevity gene, is greatly hampered by the fact that its exonic regions have 99% sequence homology with the [[FOXO3B]] pseudogene. If unaccounted for, this high degree of homology can cause serious genotyping or sequencing errors. Here, we present an experimental set-up that allows reliable data generation for the highly homologous regions and that can be used for the evaluation of assay specificity. Using this design, we exemplarily showed FOXO3A-specific results for two single-nucleotide polymorphisms (SNPs) (rs4945816 and rs4946936) that are significantly associated with longevity in our centenarian-control sample (P(each)=0.0008). Because both SNPs are located in the 3&#039; untranslated region of FOXO3A, they could be of functional relevance for the longevity phenotype. Our experimental set-up can be used for reliable and reproducible data generation for further sequencing and genotyping studies of FOXO3A with the aim of discovering new SNPs of functional relevance.<br /> |mesh-terms=* Forkhead Box Protein O3<br /> * Forkhead Transcription Factors<br /> * Genotype<br /> * Humans<br /> * Longevity<br /> * Phenotype<br /> * Polymorphism, Single Nucleotide<br /> * Pseudogenes<br /> * Sequence Homology<br /> <br /> |full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548564<br /> }}<br /> {{medline-entry<br /> |title=FOXO3 gene variants and human aging: coding variants may not be key players.<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/22459618<br /> |abstract=FOXO3 is generally recognized as a &quot;master&quot; gene in aging since its association with longevity has been replicated in multiple organisms and human populations. A group of single nucleotide polymorphisms in linkage disequilibrium with a coding region has been associated with human longevity, but the actual functional variant is unidentified. Therefore, we sequenced the coding region in our long-lived Japanese American population in order to enhance resources for fine mapping this region. We demonstrate that of 38 published variants, 6 are misalignments with homologous nonallelic sequences from [[FOXO3B]] ([[ZNF286B]]), a pseudogene on a different chromosome; 2 are attributable to [[ZNF286B]] only, and the remaining 30 were unconfirmed, indicating that they are very rare and not likely involved in longevity. Furthermore, we identified a novel, unique, nonsynonymous coding variant in exon 3 (Gly566Ala; rs138174682) that is prevalent in multiple ethnic groups but appeared too rare for major longevity effects in our study populations.<br /> |mesh-terms=* Aged<br /> * Aged, 80 and over<br /> * Aging<br /> * Alleles<br /> * Asian Americans<br /> * Case-Control Studies<br /> * Chromosome Mapping<br /> * Cohort Studies<br /> * European Continental Ancestry Group<br /> * Female<br /> * Forkhead Box Protein O3<br /> * Forkhead Transcription Factors<br /> * Genetic Variation<br /> * Humans<br /> * Longevity<br /> * Male<br /> * Middle Aged<br /> * Open Reading Frames<br /> * Polymorphism, Single Nucleotide<br /> * Sensitivity and Specificity<br /> <br /> |full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668389<br /> }}</div>

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