https://transhumanist.ru/index.php?action=history&feed=atom&title=CSRP2CSRP2 - История изменений2026-04-09T16:16:28ZИстория изменений этой страницы в викиMediaWiki 1.43.6https://transhumanist.ru/index.php?title=CSRP2&diff=5153&oldid=prevOdysseusBot: Новая страница: «Cysteine and glycine-rich protein 2 (Cysteine-rich protein 2) (CRP2) (LIM domain only protein 5) (LMO-5) (Smooth muscle cell LIM protein) (SmLIM) [LMO5] [SMLIM]...»2021-05-12T13:31:32Z<p>Новая страница: «Cysteine and glycine-rich protein 2 (Cysteine-rich protein 2) (CRP2) (LIM domain only protein 5) (LMO-5) (Smooth muscle cell LIM protein) (SmLIM) [LMO5] [SMLIM]...»</p>
<p><b>Новая страница</b></p><div>Cysteine and glycine-rich protein 2 (Cysteine-rich protein 2) (CRP2) (LIM domain only protein 5) (LMO-5) (Smooth muscle cell LIM protein) (SmLIM) [LMO5] [SMLIM]<br />
<br />
==Publications==<br />
<br />
{{medline-entry<br />
|title=Identification of a CArG-independent region of the cysteine-rich protein 2 promoter that directs expression in the developing vasculature.<br />
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/12791591<br />
|abstract=Cysteine-rich protein ([[CRP]])2 is a member of the LIM-only [[CRP]] family that is expressed in vascular smooth muscle cells (VSMC). To gain insight into the transcription of [[CSRP2]] (gene name for [[CRP]]2) in VSMC, we analyzed the 5'-flanking sequence of the [[CSRP2]] gene. We showed previously that 4,855 bp of the 5'-flanking sequence of the [[CSRP2]] gene directed lacZ reporter gene expression, primarily in the VSMC of transgenic mice. To further define the regulatory sequences important for [[CSRP2]] expression in VSMC, a series of promoter constructs containing deletions of the 5'-flanking sequence upstream of a nuclear-localized lacZ reporter gene were generated and analyzed. Similar to that observed in the -4855[[CSRP2]]-lacZ mice, beta-galactosidase reporter activity was detected in the developing great vessels, aorta, intersegmental arteries, umbilical vessels, endocardial cushions, and neural tube in the -3513-, -2663-, -795-, and -664[[CSRP2]]-lacZ lines. However, an internal deletion of bp -573 to -550 abolished the vascular, but not the neural tube, staining. Interestingly, no CArG box [CC(A/T)6GG] was present in the -795-bp fragment. Cotransfection experiments showed that dominant-negative serum response factor ([[SRF]]) did not repress [[CSRP2]] promoter activity, which was different from the repressive effect of dominant-negative [[SRF]] on the SM22 alpha promoter. Our data suggest the presence of a VSMC-specific element(s) within bp -573 to -550 of the [[CSRP2]] 5'-flanking sequence; however, in contrast to many other smooth muscle genes, transcriptional regulation of the [[CSRP2]] gene is not dependent on [[SRF]].<br />
|mesh-terms=* Aging<br />
* Animals<br />
* Base Sequence<br />
* Blood Vessels<br />
* Cell Cycle Proteins<br />
* Cells, Cultured<br />
* DNA-Binding Proteins<br />
* Embryo, Mammalian<br />
* Embryonic and Fetal Development<br />
* Gene Expression<br />
* Heterogeneous-Nuclear Ribonucleoprotein Group A-B<br />
* Male<br />
* Mice<br />
* Mice, Transgenic<br />
* Muscle, Smooth, Vascular<br />
* Myocytes, Smooth Muscle<br />
* Nuclear Proteins<br />
* Promoter Regions, Genetic<br />
* Protein Structure, Tertiary<br />
* Proteins<br />
* Rats<br />
* Rats, Sprague-Dawley<br />
* Repressor Proteins<br />
* Ribonucleoproteins<br />
* Transcription Factors<br />
* Transgenes<br />
<br />
|full-text-url=https://sci-hub.do/10.1152/ajpheart.00165.2003<br />
}}</div>OdysseusBot