https://transhumanist.ru/index.php?action=history&feed=atom&title=CLTA 2026-06-09T23:27:03Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=CLTA&diff=5378&oldid=prev 2021-05-12T13:41:24Z

<p>Новая страница: «Clathrin light chain A (Lca) ==Publications== {{medline-entry |title=Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytes....»</p> <p><b>Новая страница</b></p><div>Clathrin light chain A (Lca)<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytes.<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/12135671<br /> |abstract=A favorable incidence and severity of graft-vs-host disease is observed in patients transplanted with banked, unrelated, HLA-mismatched umbilical cord blood (UCB) grafts, while the incidence of malignant relapse remains low. CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells. T cells were isolated from UCB and adult peripheral blood and stimulated with anti-CD3 and anti-[[CD28]] monoclonal antibodies. Cells were immunostained and analyzed by flow cytometry for both surface and intracellular expression of CTLA-4 in the presence and absence of cyclosporin A, and kinetics of CTLA-4 expression compared. CTLA-4 mRNA expression was measured using quantitative real-time polymerase chain reaction. NFAT1 protein levels were measured by Western blot analysis. These studies demonstrate reduced surface and intracellular expression of CTLA-4 in stimulated UCB T cells compared to adult controls. Furthermore, reduced CTLA-4 protein expression in UCB T cells was noted to be in part transcriptionally regulated, as CTLA-4 mRNA levels also were significantly lower. Reduced [[CLTA]]-4 expression by UCB T cells followed the kinetics of delayed and reduced expression of the transcription factor NFAT1 by UCB T lymphocytes during primary stimulation. Moreover, cyclosporin A, which is known to modulate NFAT activation, reduced CTLA-4 protein expression in adult and UCB T cells. Reduced expression of the key regulatory proteins CTLA-4 and NFAT-1 may contribute to favorable UCB T lymphocyte allogeneic responses.<br /> |mesh-terms=* Abatacept<br /> * Adult<br /> * Aging<br /> * Antigens, CD<br /> * Antigens, Differentiation<br /> * CTLA-4 Antigen<br /> * Cell Division<br /> * Cyclosporine<br /> * DNA-Binding Proteins<br /> * Fetal Blood<br /> * Flow Cytometry<br /> * Gene Expression Regulation<br /> * Graft vs Host Disease<br /> * Hematopoietic Stem Cell Transplantation<br /> * Humans<br /> * Immune Tolerance<br /> * Immunoconjugates<br /> * Immunosuppressive Agents<br /> * Infant, Newborn<br /> * Lymphocyte Activation<br /> * NFATC Transcription Factors<br /> * Nuclear Proteins<br /> * Polymerase Chain Reaction<br /> * RNA, Messenger<br /> * T-Lymphocytes<br /> * Transcription Factors<br /> * Transcription, Genetic<br /> <br /> |full-text-url=https://sci-hub.do/10.1016/s0301-472x(02)00831-7<br /> }}</div>

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