https://transhumanist.ru/index.php?action=history&feed=atom&title=CLK1 2026-06-18T05:06:24Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=CLK1&diff=5157&oldid=prev 2021-05-12T13:31:42Z

<p>Новая страница: «Dual specificity protein kinase CLK1 (EC 2.7.12.1) (CDC-like kinase 1) [CLK] ==Publications== {{medline-entry |title=Conservation of the Caenorhabditis elegans...»</p> <p><b>Новая страница</b></p><div>Dual specificity protein kinase CLK1 (EC 2.7.12.1) (CDC-like kinase 1) [CLK]<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human: a gene required for ubiquinone biosynthesis with potential implications for aging.<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/10501970<br /> |abstract=Mutations in the Caenorhabditis elegans gene clk-1 have a major effect on slowing development and increasing life span. The Saccharomyces cerevisiae homolog [[COQ7]] encodes a mitochondrial protein involved in ubiquinone biosynthesis and, hence, is required for respiration and gluconeogenesis. In this study, RT-PCR and 5&#039; RACE were used to isolate both human and mouse clk-1/[[COQ7]] homologs. Human CLK-1 was mapped to Chr 16(p12-13.1) by Radiation Hybrid (RH) and fluorescence in situ hybridization (FISH) methods. The number and location of human [[CLK1]] introns were determined, and the location of introns II and IV are the same as in C. elegans. Northern blot analysis showed that three different isoforms of CLK-1 mRNA are present in several tissues and that the isoforms differ in the amount of expression. The functional equivalence of human CLK-1 to the yeast [[COQ7]] homolog was tested by introducing either a single or multicopy plasmid containing human CLK-1 cDNA into yeast coq7 deletion strains and assaying for growth on a nonfermentable carbon source. The human CLK-1 gene was able to functionally complement yeast coq7 deletion mutants. The protein similarities and the conservation of function of the CLK-1/clk-1/[[COQ7]] gene products suggest a potential link between the production of ubiquinone and aging.<br /> |mesh-terms=* Aging<br /> * Alternative Splicing<br /> * Amino Acid Sequence<br /> * Animals<br /> * Biological Clocks<br /> * Caenorhabditis elegans<br /> * Caenorhabditis elegans Proteins<br /> * Chromosome Mapping<br /> * Chromosomes, Human, Pair 16<br /> * Cloning, Molecular<br /> * Conserved Sequence<br /> * Evolution, Molecular<br /> * Exons<br /> * Genetic Complementation Test<br /> * Helminth Proteins<br /> * Humans<br /> * In Situ Hybridization, Fluorescence<br /> * Introns<br /> * Mice<br /> * Molecular Sequence Data<br /> * RNA, Messenger<br /> * Sequence Homology, Amino Acid<br /> * Ubiquinone<br /> * Yeasts<br /> <br /> |full-text-url=https://sci-hub.do/10.1007/s003359901147<br /> }}</div>

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