https://transhumanist.ru/index.php?action=history&feed=atom&title=ADD2 2026-04-05T08:48:27Z История изменений этой страницы в вики MediaWiki 1.43.6 https://transhumanist.ru/index.php?title=ADD2&diff=5820&oldid=prev 2021-05-12T14:54:18Z

<p>Новая страница: «Beta-adducin (Erythrocyte adducin subunit beta) [ADDB] ==Publications== {{medline-entry |title=SerThr-PhosphoProteome of Brain from Aged <a href="/index.php?title=PINK1&amp;action=edit&amp;redlink=1" class="new" title="PINK1 (страница не существует)">PINK1</a>-KO A53T-SNC...»</p> <p><b>Новая страница</b></p><div>Beta-adducin (Erythrocyte adducin subunit beta) [ADDB]<br /> <br /> ==Publications==<br /> <br /> {{medline-entry<br /> |title=SerThr-PhosphoProteome of Brain from Aged [[PINK1]]-KO A53T-[[SNCA]] Mice Reveals pT1928-[[MAP1B]] and pS3781-[[ANK2]] Deficits, as Hub between Autophagy and Synapse Changes.<br /> |pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31277379<br /> |abstract=Hereditary Parkinson&#039;s disease (PD) can be triggered by an autosomal dominant overdose of alpha-Synuclein ([[SNCA]]) as stressor or the autosomal recessive deficiency of [[PINK1]] Serine/Threonine-phosphorylation activity as stress-response. We demonstrated the combination of [[PINK1]]-knockout with overexpression of [[SNCA]] in double mutant (DM) mice to exacerbate locomotor deficits and to reduce lifespan. To survey posttranslational modifications of proteins underlying the pathology, brain hemispheres of old DM mice underwent quantitative label-free global proteomic mass spectrometry, focused on Ser/Thr-phosphorylations. As an exceptionally strong effect, we detected &gt;300-fold reductions of phosphoThr1928 in [[MAP1B]], a microtubule-associated protein, and a similar reduction of phosphoSer3781 in [[ANK2]], an interactor of microtubules. [[MAP1B]] depletion is known to trigger perturbations of microtubular mitochondria trafficking, neurite extension, and synaptic function, so it was noteworthy that relevantly decreased phosphorylation was also detected for other microtubule and microfilament factors, namely [[MAP2]] , [[MARK1]] , [[MAP1A]] , [[KIF1A]] , 4.1N , 4.1G , and [[ADD2]] . While the [[MAP1B]] heavy chain supports regeneration and growth cones, its light chain assists [[DAPK1]]-mediated autophagy. Interestingly, relevant phosphorylation decreases of [[DAPK2]] , [[VPS13D]] , and [[VPS13C]] in the DM brain affected regulators of autophagy, which are implicated in PD. Overall, significant downregulations were enriched for PFAM [[C2]] domains, other kinases, and synaptic transmission factors upon automated bioinformatics, while upregulations were not enriched for selective motifs or pathways. Validation experiments confirmed the change of LC3 processing as reflection of excessive autophagy in DM brain, and dependence of [[ANK2]]/[[MAP1B]] expression on [[PINK1]] levels. Our new data provide independent confirmation in a mouse model with combined PARK1/PARK4/PARK6 pathology that [[MAP1B]]/[[ANK2]] phosphorylation events are implicated in Parkinsonian neurodegeneration. These findings expand on previous observations in [i]Drosophila melanogaster[/i] that the [[MAP1B]] ortholog futsch in the presynapse is a primary target of the PARK8 protein [[LRRK2]], and on a report that [[MAP1B]] is a component of the pathological Lewy body aggregates in PD patient brains. Similarly, [i][[ANK2]][/i] gene locus variants are associated with the risk of PD, [[ANK2]] interacts with [[PINK1]]/Parkin-target proteins such as MIRO1 or [[ATP1A2]], and [[ANK2]]-derived peptides are potent inhibitors of autophagy.<br /> |mesh-terms=* Aging<br /> * Amino Acid Sequence<br /> * Animals<br /> * Ankyrins<br /> * Autophagy<br /> * Brain<br /> * Mice, Knockout<br /> * Mice, Mutant Strains<br /> * Microtubule-Associated Proteins<br /> * Microtubules<br /> * Phosphoproteins<br /> * Phosphorylation<br /> * Phosphoserine<br /> * Phosphothreonine<br /> * Protein Domains<br /> * Protein Kinases<br /> * Proteome<br /> * Synapses<br /> * alpha-Synuclein<br /> |keywords=* PINK1<br /> * Parkinson’s disease<br /> * alpha-synuclein<br /> * autophagy<br /> * brain phosphorylome<br /> * microtubular cytoskeleton<br /> * synaptic signaling<br /> |full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651490<br /> }}</div>

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