Редактирование: ULK2

Serine/threonine-protein kinase ULK2 (EC 2.7.11.1) (Unc-51-like kinase 2) [KIAA0623]

==Publications==

{{medline-entry
|title=miR-26a suppresses autophagy in swine Sertoli cells by targeting [[ULK2]].
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/29761550
|abstract=A large number of microRNAs (miRNAs) have been detected from porcine testicular tissues thanks to the development of high-throughput sequencing technology. However, the regulatory roles of most identified miRNAs in swine testicular development or spermatogenesis are poorly understood. In our previous study, [[ULK2]] (uncoordinated-51-like kinase 2) was predicted as a target gene of miR-26a. In this study, we aimed to investigate the role of miR-26a in swine Sertoli cell autophagy. The relative expression of miR-26a and [[ULK2]] levels has a significant negative correlation (R   = .5964, p ≤ .01) in nine developmental stages of swine testicular tissue. Dual-luciferase reporter assay results show that miR-26a directly targets the 3'UTR of the [[ULK2]] gene (position 618-624). In addition, both the mRNA and protein expression of [[ULK2]] were downregulated by miR-26a in swine Sertoli cells. These results indicate that miR-26a targets the [[ULK2]] gene and downregulates its expression in swine Sertoli cells. Based on the expression of marker genes (LC3, p62 and Beclin-1), overexpression of miR-26a or knock-down of [[ULK2]] inhibits swine Sertoli cell autophagy. Taken together, these findings demonstrate that miR-26a suppresses autophagy in swine Sertoli cells by targeting [[ULK2]].
|mesh-terms=* Aging
* Animals
* Autophagy
* Gene Expression Regulation, Developmental
* Gene Expression Regulation, Enzymologic
* Gene Knockdown Techniques
* Male
* MicroRNAs
* Protein-Serine-Threonine Kinases
* Sertoli Cells
* Swine
|keywords=* 
ULK2

* autophagy
* expression
* miR-26a
* swine Sertoli cells
|full-text-url=https://sci-hub.do/10.1111/rda.13177
}}