Редактирование: TINF2

TERF1-interacting nuclear factor 2 (TRF1-interacting nuclear protein 2) [TIN2]

==Publications==

{{medline-entry
|title=Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/29055871
|abstract=Loss of function of dyskerin ([[DKC1]]), [[NOP10]] and TIN2 are responsible for different inheritance patterns of Dyskeratosis congenita (DC; ORPHA1775). They are key components of telomerase ([[DKC1]] and [[NOP10]]) and shelterin (TIN2), and play an important role in telomere homeostasis. They participate in several fundamental cellular processes by contributing to Dyskeratosis congenita through mechanisms that are not fully understood. Presence of oxidative stress was postulated to result from telomerase ablation. However, the resulting disturbed redox status can promote telomere attrition by generating a vicious circle, which promotes cellular senescence. This fact prompted us to study if acute loss of [[DKC1]], [[NOP10]] and [[TINF2]] can promote redox disequilibrium as an early event when telomere shortening has not yet taken place. We generated siRNA-mediated ([[DKC1]], [[NOP10]] and [[TINF2]]) cell lines by RNA interference, which was confirmed by mRNA and protein expression analyses. No telomere shortening occurred in any silenced cell line. Depletion of H/ACA ribonucleoproteins [[DKC1]] and [[NOP10]] diminished telomerase activity via TERC down-regulation, and produced alterations in pseudouridylation and ribosomal biogenesis. An increase in the GSSG/GSH ratio, carbonylated proteins and oxidized peroxiredoxin-6 was observed, in addition to MnSOD and TRX1 overexpression in the siRNA DC cells. Likewise, high PARylation levels and high [[PARP1]] protein expression were detected. In contrast, the silenced [[TINF2]] cells did not alter any evaluated oxidative stress marker. Altogether these findings lead us to conclude that loss of [[DKC1]] and [[NOP10]] functions induces oxidative stress in a telomere shortening independent manner.
|mesh-terms=* Cell Cycle Proteins
* Cellular Senescence
* DNA Damage
* Dyskeratosis Congenita
* HeLa Cells
* Humans
* Nuclear Proteins
* Oxidative Stress
* RNA Interference
* Ribonucleoproteins, Small Nucleolar
* Telomerase
* Telomere Shortening
* Telomere-Binding Proteins
|keywords=* Aging
* Antioxidant
* DNA damage
* Oxidative stress
* Telomeropathies
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650655
}}