Редактирование: SSX2

Protein SSX2 (Cancer/testis antigen 5.2) (CT5.2) (Synovial sarcoma, X breakpoint 2) (Tumor antigen HOM-MEL-40) [SSX2A]

==Publications==

{{medline-entry
|title=Ectopic expression of cancer/testis antigen [[SSX2]] induces DNA damage and promotes genomic instability.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/25363656
|abstract=SSX cancer/testis antigens are frequently expressed in melanoma tumors and represent attractive targets for immunotherapy, but their role in melanoma tumorigenesis has remained elusive. Here, we investigated the cellular effects of [[SSX2]] expression. In A375 melanoma cells, [[SSX2]] expression resulted in an increased DNA content and enlargement of cell nuclei, suggestive of replication aberrations. The cells further displayed signs of DNA damage and genomic instability, associated with p53-mediated G1 cell cycle arrest and a late apoptotic response. These results suggest a model wherein [[SSX2]]-mediated replication stress translates into mitotic defects and genomic instability. Arrest of cell growth and induction of DNA double-strand breaks was also observed in MCF7 breast cancer cells in response to [[SSX2]] expression. Additionally, MCF7 cells with ectopic [[SSX2]] expression demonstrated typical signs of senescence (i.e. an irregular and enlarged cell shape, enhanced β-galactosidase activity and DNA double-strand breaks). Since replication defects, DNA damage and senescence are interconnected and well-documented effects of oncogene expression, we tested the oncogenic potential of [[SSX2]]. Importantly, knockdown of [[SSX2]] expression in melanoma cell lines demonstrated that [[SSX2]] supports the growth of melanoma cells. Our results reveal two important phenotypes of ectopic [[SSX2]] expression that may drive/support tumorigenesis: First, immediate induction of genomic instability, and second, long-term support of tumor cell growth.
|mesh-terms=* Cell Line, Tumor
* Cellular Senescence
* DNA Breaks, Double-Stranded
* G1 Phase Cell Cycle Checkpoints
* Gene Expression Regulation, Neoplastic
* Genomic Instability
* Humans
* Melanoma
* Neoplasm Proteins
* Repressor Proteins
* Tumor Suppressor Protein p53
|keywords=* Cancer/testis antigen
* Genomic instability
* Oncogene
* SSX2
* Senescence
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5528659
}}