Редактирование: MAN2B2

Epididymis-specific alpha-mannosidase precursor (EC 3.2.1.24) (Alpha-1,6-mannosidase) (Mannosidase alpha class 2B member 2) [KIAA0935]

==Publications==

{{medline-entry
|title=Refinement of the expression pattern of a mouse homologue of the porcine 135-kDa alpha-d-mannosidase ([[MAN2B2]]).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/10860856
|abstract=In the article by Hiramoto et al. (1997) (Stage-specific expression of a mouse homologue of the porcine 135 kDa alpha-d-mannosidase ([[MAN2B2]]) in type A spermatogonia, Biochem. Biophys. Res. Commun. 241, 439-445) a new homologue to the porcine epididymis-specific 135 kDa alpha-d-mannosidase ([[MAN2B2]]) was cloned from a mouse testis cDNA library. Northern blot analysis with RNA of different tissues showed a testis-specific expression by using a mouse [[MAN2B2]] oligonucleotide. In situ hybridization revealed that the mouse [[MAN2B2]] transcript is localized exclusively in spermatogonia. In consequence to this it was proposed by Hiramoto et al. that the mouse [[MAN2B2]] homologue could serve as a marker for spermatogonia. By repeating the published experiments we observed a different expression pattern of the mouse [[MAN2B2]] gene. Northern blot analysis with either testicular RNA from prepubertal males or with testicular RNA from the W/W(v)-mutant mouse which lacks germ cells showed an expression of the [[MAN2B2]] gene. Furthermore, Northern blot analysis with RNA from different somatic tissues revealed that the gene is ubiquitously expressed. Therefore, our refinement clearly demonstrates that the [[MAN2B2]] mouse homologue is not specifically expressed in spermatogonia.
|mesh-terms=* Aging
* Animals
* Blotting, Northern
* DNA Probes
* Gene Expression Profiling
* Gene Expression Regulation, Developmental
* Male
* Mannosidases
* Mice
* Mice, Mutant Strains
* Molecular Weight
* Organ Specificity
* RNA, Messenger
* Reproducibility of Results
* Sequence Homology
* Sexual Maturation
* Spermatozoa
* Swine
* Testis
* alpha-Mannosidase

|full-text-url=https://sci-hub.do/10.1006/bbrc.2000.2868
}}