Редактирование: KCNE2

Potassium voltage-gated channel subfamily E member 2 (MinK-related peptide 1) (Minimum potassium ion channel-related peptide 1) (Potassium channel subunit beta MiRP1)

==Publications==

{{medline-entry
|title=[[[KCNE2]] protein S98 phosphorylation in heart of old SHR rats detected by point mutagenesis].
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/17717828
|abstract=To investigate the phosphorylation of [[KCNE2]] protein in heart of old SHR rats. The membrane proteins from ventricular myocardium of old SHR were extracted, treated with or without alkaline phosphatase and tested binding with Ab2 (an anti-[[KCNE2]] polyclonal antibody) by Western blot. A [[KCNE2]] fusion protein with c-myc was obtained from in vitro translation system and treated with or without alkaline phosphatase. A series of mono- and double-point mutated fusion [[KCNE2]] proteins with c-myc were obtained from an in vitro translation system, and Western blots with Ab2 or anti-myc antibody were performed. After alkaline phosphatase treatment, Ab2 significantly attenuated its binding with [[KCNE2]]. In vitro translation system confirmed that after alkaline phosphatase treatment, Ab2 weakened binding ability to [[KCNE2]] while binding to c-myc was not changed. Point mutation experiments showed that serine residue in position 98 of [[KCNE2]] proteins might be phosphorylated. [[KCNE2]] protein in heart of old SHR rats is phosphorylated and this phosphorylation takes place in serine residue of position 98.
|mesh-terms=* Aging
* Animals
* Blotting, Western
* Hypertension
* Myocardium
* Phosphorylation
* Point Mutation
* Potassium Channels, Voltage-Gated
* Protein Binding
* Proto-Oncogene Proteins c-myc
* Rats
* Rats, Inbred SHR
* Recombinant Fusion Proteins


}}
{{medline-entry
|title=[[KCNE2]] protein is expressed in ventricles of different species, and changes in its expression contribute to electrical remodeling in diseased hearts.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/15066947
|abstract=Mutations in [[KCNE2]] have been linked to long-QT syndrome (LQT6), yet [[KCNE2]] protein expression in the ventricle and its functional role in native channels are not clear. We detected [[KCNE2]] protein in human, dog, and rat ventricles in Western blot experiments. Immunocytochemistry confirmed [[KCNE2]] protein expression in ventricular myocytes. To explore the functional role of [[KCNE2]], we studied how its expression was altered in 2 models of cardiac pathology and whether these alterations could help explain observed changes in the function of native channels, for which [[KCNE2]] is a putative auxiliary (beta) subunit. In canine ventricle injured by coronary microembolizations, the rapid delayed rectifier current (I(Kr)) density was increased. Although the protein level of [[ERG]] (I(Kr) pore-forming, alpha, subunit) was not altered, the [[KCNE2]] protein level was markedly reduced. These data are consistent with the effect of heterologously expressed [[KCNE2]] on [[ERG]] and suggest that in canine ventricle, [[KCNE2]] may associate with [[ERG]] and suppress its current amplitude. In aging rat ventricle, the pacemaker current (I(f)) density was increased. There was a significant increase in the [[KCNE2]] protein level, whereas changes in the alpha-subunit ([[HCN2]]) were not significant. These data are consistent with the effect of heterologously expressed [[KCNE2]] on [[HCN2]] and suggest that in aging rat ventricle, [[KCNE2]] may associate with [[HCN2]] and enhance its current amplitude. [[KCNE2]] protein is expressed in ventricles, and it can play diverse roles in ventricular electrical activity under (patho)physiological conditions.
|mesh-terms=* Aging
* Animals
* COS Cells
* Chlorocebus aethiops
* Disease Models, Animal
* Dogs
* ERG1 Potassium Channel
* Ether-A-Go-Go Potassium Channels
* Heart Conduction System
* Heart Ventricles
* Humans
* Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
* Ion Channels
* Ion Transport
* Long QT Syndrome
* Macromolecular Substances
* Male
* Muscle Proteins
* Myocardial Ischemia
* Myocardium
* Potassium
* Potassium Channels
* Potassium Channels, Voltage-Gated
* Protein Subunits
* Rats
* Rats, Inbred SHR
* Rats, Inbred WKY
* Species Specificity
* Transfection
* Ventricular Remodeling

|full-text-url=https://sci-hub.do/10.1161/01.CIR.0000124225.43852.50
}}