Редактирование: DUSP8

Dual specificity protein phosphatase 8 (EC 3.1.3.16) (EC 3.1.3.48) (Dual specificity protein phosphatase hVH-5) [C11orf81] [VH5]

==Publications==

{{medline-entry
|title=MiR-21-5p/dual-specificity phosphatase 8 signalling mediates the anti-inflammatory effect of haem oxygenase-1 in aged intracerebral haemorrhage rats.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31400088
|abstract=Intracerebral haemorrhage (ICH) is a severe neurological disorder caused by bleeding within the brain tissue. Inflammation has been implicated in ICH pathogenesis and is a potential therapeutic target for ICH. Haemin, an activator of haem oxygenase-1 (HO-1), rapidly increases HO-1 protein expression and activity and has been shown to distinctly affect anti-inflammatory functions after central nervous system (CNS) injury. However, less is known about the mechanisms that underlie the anti-inflammatory effects of haemin in aged rats post-ICH. Here, we performed microarray analysis to identify miRNAs that respond strongly to HO-1 regulation in ICH rats and found that miR-21-5p induced the most significant change. Using Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and Gene Ontology (GO) analysis, we focused on dual-specificity phosphatase 8 ([[DUSP8]]) from the predicted miR-21-5p targets. Luciferase reporter assays confirmed that miR-21-5p bound directly to [[DUSP8]]. MiR-21-5p upregulation in vitro downregulated [[DUSP8]] expression. Importantly, intracerebroventricularly injecting antagomir for miR-21-5p (A-miR-21-5p), which was used to inhibit miR-21-5p in aged ICH rats, significantly reduced the neurological defects, repaired cognitive impairment, alleviated blood-brain barrier (BBB) permeability, inhibited neuronal apoptosis posthaemorrhage and accelerated haematoma absorption. In addition, serum miR-21-5p levels were notably elevated in patients relative to healthy individuals and were correlated with National Institutes of Health Stroke Scale (NIHSS) scores and clinical outcomes. In summary, A-miR-21-5p increased HO-1 expression in cerebral haematomas, thus eliciting the [[DUSP8]]-modulated perifocal neuroprotective effect of haemin. MiR-21-5p with haemin therapy may be a potential therapy post-ICH.
|mesh-terms=* Aging
* Animals
* Antagomirs
* Anti-Inflammatory Agents
* Cells, Cultured
* Cerebral Hemorrhage
* Dual-Specificity Phosphatases
* HEK293 Cells
* Heme Oxygenase-1
* Hemin
* Humans
* Male
* MicroRNAs
* Rats
* Rats, Sprague-Dawley
* Signal Transduction
|keywords=* aging
* dual-specificity phosphatase 8
* haem oxygenase-1
* intracerebral haemorrhage
* microRNA
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826124
}}