Редактирование: Whole brain emulation (раздел)

=== KESM ===

[[File:KESM.jpg|thumb|right|The Knife Edge Scanning Microscope - KESM. Texas A&M University.]]

The Knife-Edge Scaning Microscope is a machine that integrates sectioning and imaging into a single process, with the diamond knife and microscope moving together in the same assembly, and can scan large volumes of tissue at high resolution (Below SBFSEM). It would allow for an entire mouse brain (≈1 cm<sup>3</sup>) to be scanned in one hundred hours, producing 15 Terabytes of raw EM data.<ref>McCormick, 2002a; McCormick, 2002b)</ref>

The main limitation is the need to stain inside a volume. The array of stains is at present limited, but genetic modifications may allow for cells to express stains or become fluorescent.

The tissue is fixed and embedded in plastic. As the knife and the microscope move over the tissue, a thin strip of tissue is cut and scanned. The KESM can scan up to 200 megapixels per second, with the width of the EM field being 2.5mm for 64μm resolution and 0.625mm for 32μm resolution<ref>McCormick, Koh et al., 2004</ref>. The sample is cut in a stair-step manner to reduce jittering and use knives larger than the microscope FOV<ref>Koh and McCormick, 2003</ref>.