Редактирование: Cryopreservation (раздел)

=== Perfusion ===


==== Vitrification ====

In essence, vitrification is the depression of the freezing point of water until it is below the Glass Transition Point (T<sub>g</sub>), at which water and the tissues around it become a glass, and ice formation cannot occur.

==== Cryoprotectants ====

A cryoprotectant is any substance that protects biological tissue from freezing damage (Ice formation).

Cryoprotectants such as polyols, sugars and glycols are naturally produced by some forms of life inside the arctic and antarctic circles to protect themselves from the winter. Some arctic salamanders produce [[#Glycerol|glycerol]] in their livers as a cryoprotectant.

Cryoprotectants can be divided into two categories: Conventional and solutions. Conventional cryoprotectants are glycols, such as [[#Ethylene glycol|Ethylene glycol]], [[#Glycerol|glycerol]]; and [[#DMSO|dimethyl sulfoxide]]. Solutions are mixtures of conventional cryoprotectants and/or other chemicals, and are designed to reduce toxicity to biological tissue and increase effectiveness.

===== M-22 =====

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===== CI-VM-1 =====

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* [http://cryonics.org/research/CI-VM-1.html More information]

===== Ethylene Glycol =====

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===== Glycerol =====

Source: [[Mike Darwin]]. ''History of DMSO and Glycerol in Cryonics''. ''Cryonics'', Third Quarter 2007.

The use of glycerol as a cryoprotectant for cryonics [[#Patients|patients]] was first proposed in the founding book of cryonics, [[#Books|The Prospect of Immortality]], given that it was the most used cryoprotectant agent at the time (1962-1964) and good preservation of sperm and tissue using glycerol had been demonstrated.

===== DMSO =====

DMSO was introduced in the mid-to-late sixties due both to its skin-penetrating, anti-inflammatory properties and the charisma of Stanley W. Jacob, the "father of Dimethyl Sulfoxide".

===== Propylene Glycol =====

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===== Tissue Comparison =====

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==== Burr Hole ====