Редактирование: Whole brain emulation (раздел)

==== Optical Histology ====

All‐optical histology uses femtosecond laser pulses to ablate tissue samples, avoiding the 
need for mechanical removal of the surface layer (Tsai, Friedman et al., 2003). This treatment 
appears to change the tissue 2‐10 μm from the surface. However, Tsai et al. were optimistic 
about being able to scan a fluorescence labelled entire mouse brain into 2 terapixels at the 
diffraction limit of spatial resolution.  
 
Another interesting application of femtosecond laser pulses is microdissection (Sakakura, 
Kajiyama et al., 2007; Colombelli, Grill et al., 2004). The laser was able to remove 100 μm 
samples from plant and animal material, modifying a ~10 μm border. This form of optical 
dissection might be an important complement for EM methods, in that, after scanning the 
geometry of the tissue at a high resolution, relevant pieces can be removed and analyzed 
microchemically. This could enable gaining both the EM connectivity data and detailed 
biochemistry information. Platforms already exist that can both inject biomolecules into 
individual cells, perform microdissection, isolate and collect individual cells using laser 
catapulting, and set up complex optical force patterns (Stuhrmann, Jahnke et al., 2006).