Редактирование: STX3 (раздел)

==Publications==

{{medline-entry
|title=Myelopoiesis in spleen-producing distinct dendritic-like cells.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/22117595
|abstract=Dendritic cells (DC) represent a heterogeneous class of antigen presenting cells (APC). Previously we reported a distinct myeloid dendritic-like cell present in spleen, as an in vivo counterpart to cells produced in murine spleen long-term cultures (LTC-DC). These cells, named 'L-DC', were found to be functionally and phenotypically distinct from conventional (c)DC, plasmacytoid (p)DC and monocytes. These results suggested that spleen may represent a niche for development of L-DC from endogenous progenitors. Adult murine spleen has now been investigated for the presence of L-DC progenitors. Lineage-negative (Lin)(-) ckit(lo)  and Lin(-) ckit(hi)  progenitor subsets were identified as candidate populations, and tested for ability to produce L-DC; in vitro upon co-culture with the spleen stromal line [[STX3]], and in vivo after adoptive therapy into mice. Both subsets colonized [[STX3]] stroma in vitro for L-DC production, indicating that they contained either a common or two distinct progenitors for L-DC. However, only the Lin(-) ckit(hi)  subset gave progeny cells after adoptive transfer into lethally irradiated mice. In vivo development was however multilineage and not restricted to L-DC development. Multilineage reconstitution reflects long-term reconstituting haematopoietic stem cells (LT-HSC), suggesting a close relationship between L-DC progenitors and LT-HSC. L-DC were however produced in vivo in much higher number than monocytes/macrophages and cDC, indicating the presence of a specific L-DC progenitor within the Lin(-) ckit(hi)  subset. A model is advanced for development of L-DC directly from haematopoietic progenitors in spleen and dependent on the spleen microenvironment.
|mesh-terms=* Aging
* Animals
* Cell Differentiation
* Cell Separation
* Coculture Techniques
* Dendritic Cells
* Hematopoietic Stem Cells
* Mice
* Mice, Inbred C57BL
* Myelopoiesis
* Spleen
* Time Factors

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822703
}}