Редактирование: SNRPN (раздел)

==Publications==

{{medline-entry
|title=Age-associated changes in gene expression of goat oocytes.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/23746875
|abstract=Oocyte aging severely decreases the quality of oocytes, which hampers fertilization and subsequent embryo development. In the present study, age-dependent molecular changes in goat oocytes were investigated. First, the quality of goat oocytes with various in vitro culture times (24, 30, 36, 48, and 60 hours) was evaluated on the basis of developmental rates of parthenogenetically activated embryos and apoptosis of cumulus cells (CCs). Second, relative gene expression of six genes (mitochondrial genes: [[PGC]]-1α and NRF-1; epigenetic modification genes: [[SNRPN]] and [[HAT1]]; mitotic spindle checkpoint protein: [[SMAD2]]; and hyaluronan synthase gene: [[HAS3]]) were analyzed during oocyte aging. Third, we further studied the changes of seven genes ([[PGC]]-1α and NRF-1; apoptotic-related genes: [[BAX]] and [[BCL2]]; hyaluronan synthase gene: [[HAS2]]; metabolism-related gene: STAR; and superoxide dismutase gene: [[SOD1]]) in CCs during oocyte aging. In these studies, the blastocyst rate gradually decreased and the number of apoptotic cells significantly increased as the culture time increased (P < 0.05). Moreover, relative gene expressions of [[PGC]]-1α, NRF-1 and [[SMAD2]] significantly decreased from 24 to 36 hours (P < 0.05), whereas the levels of [[HAT1]] and [[HAS3]] slowly increased as culture was prolonged. Furthermore, the levels of [[PGC]]-1α, [[BCL2]], [[HAS2]] and [[SOD1]] quickly reduced, and [[BAX]] significantly increased from 24 to 36 hours in aged CCs (P < 0.05). In conclusion, goat oocytes started to age at 30 hours in vitro culture, and gene expression patterns of oocytes and CCs significantly changed as the oocytes aged. Gene expression pattern changes in CCs may provide a convenient and effective way to detect oocyte aging without compromising oocyte integrity.
|mesh-terms=* Aging
* Animals
* Apoptosis
* Cells, Cultured
* Cellular Senescence
* Female
* Gene Expression
* Goats
* Oocytes
* Parthenogenesis
* Time Factors
|keywords=* Aging
* Apoptosis
* Cumulus cells
* Gene expression
* Goat
* Oocytes
|full-text-url=https://sci-hub.do/10.1016/j.theriogenology.2013.04.019
}}