Редактирование: SLPI (раздел)

==Publications==

{{medline-entry
|title=Human buccal epithelium acquires microbial hyporesponsiveness at birth, a role for secretory leukocyte protease inhibitor.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/25056659
|abstract=Repetitive interaction with microbial stimuli renders epithelial cells (ECs) hyporesponsive to microbial stimulation. Previously, we have reported that buccal ECs from a subset of paediatric patients with Crohn's disease are not hyporesponsive and spontaneously released chemokines. We now aimed to identify kinetics and mechanisms of acquisition of hyporesponsiveness to microbial stimulation using primary human buccal epithelium. Buccal ECs collected directly after birth and in later stages of life were investigated. Chemokine release and regulatory signalling pathways were studied using primary buccal ECs and the buccal EC line TR146. Findings were extended to the intestinal mucosa using murine model systems. Directly after birth, primary human buccal ECs spontaneously produced the chemokine CXCL-8 and were responsive to microbial stimuli. Within the first weeks of life, these ECs attained hyporesponsiveness, associated with inactivation of the NF-κB pathway and upregulation of the novel NF-κB inhibitor [[SLPI]] but no other known NF-κB inhibitors. [[SLPI]] protein was abundant in the cytoplasm and the nucleus of hyporesponsive buccal ECs. Knock-down of [[SLPI]] in TR146-buccal ECs induced loss of hyporesponsiveness with increased NF-κB activation and subsequent chemokine release. This regulatory mechanism extended to the intestine, as colonisation of germfree mice elicited [[SLPI]] expression in small intestine and colon. Moreover, [[SLPI]]-deficient mice had increased chemokine expression in small intestinal and colonic ECs. We identify [[SLPI]] as a new player in acquisition of microbial hyporesponsiveness by buccal and intestinal epithelium in the first weeks after microbial colonisation.
|mesh-terms=* Adult
* Aging
* Animals
* Cells, Cultured
* Chemokine CXCL2
* Down-Regulation
* Epithelium
* Gene Knockdown Techniques
* Humans
* Immune Tolerance
* Infant
* Infant, Newborn
* Interleukin-8
* Intestinal Mucosa
* Mice
* Middle Aged
* Mouth Mucosa
* NF-kappa B
* Peptidoglycan
* Secretory Leukocyte Peptidase Inhibitor
|keywords=* Bacterial Interactions
* Epithelial Cells
* Gut Immunology
* IBD Basic Research
|full-text-url=https://sci-hub.do/10.1136/gutjnl-2013-306149
}}