Редактирование: PPP2R3C (раздел)

==Publications==

{{medline-entry
|title=The mir-465 family is upregulated with age and attenuates growth hormone signaling in mouse liver.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30637918
|abstract=We analyzed the small RNA transcriptome from 5-month-old, 24-month-old, and 36-month-old mouse liver and found 56 miRNAs that changed their expression profile with age. Among these is a cluster of 18 miRNAs that are upregulated between 50- and 1,000-fold at 24 and 36 months of age. This cluster is located in a 60-kb region of the X-chromosome that is devoid of other coding sequences and is part of a lamin-associated domain. Potential targets of the miRNAs in the cluster suggest they may regulate several pathways altered in aging, including the PI3K-Akt pathway. Total transcriptome analyses indicate that expression of several potential genes in the PI3K-Akt pathway that may be targeted by the mir-465 family (mmu-mir-465a, mmu-mir-465b, and mmu-mir-465c) is downregulated with age. Transfection of the liver cell line AML12 with mir-465 family members leads to a reduction of three of these potential targets at the mRNA level: a 40% reduction of the growth hormone receptor ([[GHR]]), and a 25% reduction in Kitl and [[PPP2R3C]]. Further investigation of the [[GHR]] 3'UTR revealed that the mir-465 family directly targets the [[GHR]] mRNA. Cells transfected with mir-465 showed a reduction of [[JAK2]] and STAT5 phosphorylation upon growth hormone (GH) stimulation, resulting in a reduction in insulin-like growth factor 1 (IGF-1) and IGF-1-binding protein 3 expression. With age, GH signaling falls and there is a reduction in circulating IGF-1. Our data suggest that an increase in expression of the mir-465 family with age may contribute to the reduction in the GH signaling.
|mesh-terms=* Aging
* Animals
* Growth Hormone
* HEK293 Cells
* High-Throughput Nucleotide Sequencing
* Humans
* Liver
* Male
* Mice
* Mice, Inbred C57BL
* MicroRNAs
* Signal Transduction
* Up-Regulation
|keywords=* aging
* growth hormone receptor
* growth hormone signaling
* liver
* miRNAs
* mouse
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413667
}}