Редактирование: GPC1 (раздел)

==Publications==

{{medline-entry
|title=Decreased expression of [[GPC1]] in human skin keratinocytes and epidermis during ageing.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31430521
|abstract=Glypicans (GPCs) are heparan sulfate cell membrane proteoglycans containing glycosylphosphatidylinositol (GPI) anchor. They play important role in cell behavior by activating/presenting numerous growth factors and cytokines. The expression of GPCs was investigated in primary culture of skin keratinocytes sampled from healthy donors of different age. Primary keratinocytes from healthy female donors aged from 20 to 89 years old (n = 30) were either isolated from breast or abdominal skin samples (n = 27) or purchased (n = 3). GPCs expression was examined by qPCR, immunohistochemistry and western blot. Its role in proliferation induced by fibroblast growth factor 2 ([[FGF2]]) was also studied. Glypican 1 ([[GPC1]]) was the major expressed GPC in human keratinocytes. Its expression was up to two orders of magnitude higher than other GPCs and was significantly decreased with the age of the donors. It was localized at the cell surface and associated with intracellular granules. In skin sections, [[GPC1]] was mainly localized in basal layer of epidermis. Shedding of GPCs decreased the proliferative effect of [[FGF2]], confirming their role of modulator of growth factor effects on keratinocytes. These results established [[GPC1]] as an important player in epidermis biology and skin ageing.
|mesh-terms=* Adult
* Aged
* Aged, 80 and over
* Aging
* Cell Proliferation
* Cells, Cultured
* Epidermis
* Female
* Fibroblast Growth Factor 2
* Gene Expression Regulation
* Glypicans
* Humans
* Keratinocytes
* Middle Aged
* RNA, Messenger
* Signal Transduction
* Skin
* Young Adult
|keywords=* Ageing
* Epidermis
* Glypican 1
* Human skin
* Keratinocytes
|full-text-url=https://sci-hub.do/10.1016/j.exger.2019.110693
}}