Редактирование: CSRP2 (раздел)

==Publications==

{{medline-entry
|title=Identification of a CArG-independent region of the cysteine-rich protein 2 promoter that directs expression in the developing vasculature.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/12791591
|abstract=Cysteine-rich protein ([[CRP]])2 is a member of the LIM-only [[CRP]] family that is expressed in vascular smooth muscle cells (VSMC). To gain insight into the transcription of [[CSRP2]] (gene name for [[CRP]]2) in VSMC, we analyzed the 5'-flanking sequence of the [[CSRP2]] gene. We showed previously that 4,855 bp of the 5'-flanking sequence of the [[CSRP2]] gene directed lacZ reporter gene expression, primarily in the VSMC of transgenic mice. To further define the regulatory sequences important for [[CSRP2]] expression in VSMC, a series of promoter constructs containing deletions of the 5'-flanking sequence upstream of a nuclear-localized lacZ reporter gene were generated and analyzed. Similar to that observed in the -4855[[CSRP2]]-lacZ mice, beta-galactosidase reporter activity was detected in the developing great vessels, aorta, intersegmental arteries, umbilical vessels, endocardial cushions, and neural tube in the -3513-, -2663-, -795-, and -664[[CSRP2]]-lacZ lines. However, an internal deletion of bp -573 to -550 abolished the vascular, but not the neural tube, staining. Interestingly, no CArG box [CC(A/T)6GG] was present in the -795-bp fragment. Cotransfection experiments showed that dominant-negative serum response factor ([[SRF]]) did not repress [[CSRP2]] promoter activity, which was different from the repressive effect of dominant-negative [[SRF]] on the SM22 alpha promoter. Our data suggest the presence of a VSMC-specific element(s) within bp -573 to -550 of the [[CSRP2]] 5'-flanking sequence; however, in contrast to many other smooth muscle genes, transcriptional regulation of the [[CSRP2]] gene is not dependent on [[SRF]].
|mesh-terms=* Aging
* Animals
* Base Sequence
* Blood Vessels
* Cell Cycle Proteins
* Cells, Cultured
* DNA-Binding Proteins
* Embryo, Mammalian
* Embryonic and Fetal Development
* Gene Expression
* Heterogeneous-Nuclear Ribonucleoprotein Group A-B
* Male
* Mice
* Mice, Transgenic
* Muscle, Smooth, Vascular
* Myocytes, Smooth Muscle
* Nuclear Proteins
* Promoter Regions, Genetic
* Protein Structure, Tertiary
* Proteins
* Rats
* Rats, Sprague-Dawley
* Repressor Proteins
* Ribonucleoproteins
* Transcription Factors
* Transgenes

|full-text-url=https://sci-hub.do/10.1152/ajpheart.00165.2003
}}