Редактирование: CSGALNACT1 (раздел)

==Publications==

{{medline-entry
|title=Alterations in the chondroitin sulfate chain in human osteoarthritic cartilage of the knee.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/24280246
|abstract=To determine whether the structure of chondroitin sulfate ([[CS]]) in cartilage is reflected by the degree of cartilage degeneration in patients with osteoarthritis (OA) of the knee and to determine how [[CS]] biosynthesis affects cartilage degeneration. Two osteoarthritic cartilage samples were obtained from medial femoral condyle (MFC) and lateral femoral condyle (LFC) of 24 knees with end-stage OA. The samples were assigned to two groups as follows: lesion and remote cartilage were adjacent to and remote from the osteoarthritic cartilage, respectively. Histological grade was determined according to the Mankin score. The [[CS]] concentration and chain length were determined using high-performance liquid chromatography (HPLC) and gel filtration chromatography, respectively. Expression of the gene encoding [[CS]] glycosyltransferase was evaluated using a real-time quantitative polymerase chain reaction (qPCR) assay. These results were compared between lesion and remote cartilage. The Mankin score indicated that lesion cartilage was more degraded compared with remote cartilage. Although the [[CS]] levels varied among individuals, the mean [[CS]] concentration and chain length were significantly lower and shorter in lesion cartilage than in remote cartilage, respectively (concentration: 12.04 vs 14.84 μg/mg wet weight, P = 0.021; chain length: 5.36 vs 6.19 kDa, P = 0.026). Three genes encoding [[CS]] glycosyltransferases (CHPF, [[[[CS]]GALNACT1]], [[CS]]GALNACT2) were expressed at lower levels in lesion cartilage. In the osteoarthritic knee, the [[CS]] concentration and chain length were reduced closer to the more degraded cartilage with decreasing [[CS]] glycosyltransferase gene expression. Inhibition of [[CS]] glycosyltransferase gene expression may reduce [[CS]] chain length, which may contribute to OA progression.
|mesh-terms=* Aged
* Aged, 80 and over
* Aging
* Cartilage, Articular
* Chondrocytes
* Chondroitin Sulfates
* Female
* Gene Expression Regulation, Enzymologic
* Glycosyltransferases
* Humans
* Joint Deformities, Acquired
* Knee Joint
* Male
* Middle Aged
* Molecular Weight
* Osteoarthritis, Knee
* RNA, Messenger
* Radiography
|keywords=* Cartilage
* Chondrocyte
* Chondroitin sulfate
* Osteoarthritis
|full-text-url=https://sci-hub.do/10.1016/j.joca.2013.11.010
}}