Редактирование: CLTA (раздел)

==Publications==

{{medline-entry
|title=Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytes.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/12135671
|abstract=A favorable incidence and severity of graft-vs-host disease is observed in patients transplanted with banked, unrelated, HLA-mismatched umbilical cord blood (UCB) grafts, while the incidence of malignant relapse remains low. CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells. T cells were isolated from UCB and adult peripheral blood and stimulated with anti-CD3 and anti-[[CD28]] monoclonal antibodies. Cells were immunostained and analyzed by flow cytometry for both surface and intracellular expression of CTLA-4 in the presence and absence of cyclosporin A, and kinetics of CTLA-4 expression compared. CTLA-4 mRNA expression was measured using quantitative real-time polymerase chain reaction. NFAT1 protein levels were measured by Western blot analysis. These studies demonstrate reduced surface and intracellular expression of CTLA-4 in stimulated UCB T cells compared to adult controls. Furthermore, reduced CTLA-4 protein expression in UCB T cells was noted to be in part transcriptionally regulated, as CTLA-4 mRNA levels also were significantly lower. Reduced [[CLTA]]-4 expression by UCB T cells followed the kinetics of delayed and reduced expression of the transcription factor NFAT1 by UCB T lymphocytes during primary stimulation. Moreover, cyclosporin A, which is known to modulate NFAT activation, reduced CTLA-4 protein expression in adult and UCB T cells. Reduced expression of the key regulatory proteins CTLA-4 and NFAT-1 may contribute to favorable UCB T lymphocyte allogeneic responses.
|mesh-terms=* Abatacept
* Adult
* Aging
* Antigens, CD
* Antigens, Differentiation
* CTLA-4 Antigen
* Cell Division
* Cyclosporine
* DNA-Binding Proteins
* Fetal Blood
* Flow Cytometry
* Gene Expression Regulation
* Graft vs Host Disease
* Hematopoietic Stem Cell Transplantation
* Humans
* Immune Tolerance
* Immunoconjugates
* Immunosuppressive Agents
* Infant, Newborn
* Lymphocyte Activation
* NFATC Transcription Factors
* Nuclear Proteins
* Polymerase Chain Reaction
* RNA, Messenger
* T-Lymphocytes
* Transcription Factors
* Transcription, Genetic

|full-text-url=https://sci-hub.do/10.1016/s0301-472x(02)00831-7
}}
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